The role of NOS1AP, a schizophrenia susceptibility gene, in the regulation of dendrite branching, dendritic spine formation, and actin dynamics.
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Hernandez, Kristina.
The role of NOS1AP, a schizophrenia susceptibility gene, in the regulation of dendrite branching, dendritic spine formation, and actin dynamics. Retrieved from
https://doi.org/doi:10.7282/T3G44S8F
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TitleThe role of NOS1AP, a schizophrenia susceptibility gene, in the regulation of dendrite branching, dendritic spine formation, and actin dynamics.
Date Created2015
Other Date2015-10 (degree)
Extent1 online resource (viii, 146 p. : ill.)
DescriptionProper communication between neurons is dependent upon the appropriate patterning of dendrites and the correct distribution and structure of spines. Schizophrenia is one of several neurodevelopmental disorders that are characterized by alterations in dendrite branching and spine density. NOS1AP is a protein encoded by a schizophrenia susceptibility gene, and its expression is upregulated in the dorsolateral prefrontal cortex of patients with schizophrenia. Previously, our laboratory showed that NOS1AP isoforms negatively regulate dendrite branching in cultured rat hippocampal neurons. Since dendrites and spines are influenced by changes in the cytoskeleton, we investigated whether the overexpression of NOS1AP isoforms in heterologous cells alters actin and microtubule organization. Overexpression of a long isoform of NOS1AP (NOS1AP-L) increases the presence of microtubule organizing centers, whereas overexpression of the short isoform of NOS1AP (NOS1AP-S) decreases microtubule organization. Furthermore, NOS1AP isoforms associate with F-actin in rat brain and can alter actin organization in distinct ways. NOS1AP-S increases actin polymerization, and its overexpression in HEK293T cells decreases total Rac1 and cofilin protein expression. To elucidate the role of NOS1AP in spine formation and synaptic function, we overexpressed NOS1AP isoforms in cultured rat cortical neurons. Overexpression of NOS1AP-L increases the number of immature spines, whereas overexpression of NOS1AP-S increases the number of mature and immature spines. In addition, overexpression of NOS1AP-S increases the frequency of miniature excitatory postsynaptic currents (mEPSCs) but not the amplitude. Overexpression of NOS1AP-L decreases the amplitude of mEPSCs but not the frequency. To investigate whether NOS1AP-L can mediate changes to dendrite patterning in vivo, we overexpressed NOS1AP-L in neuronal progenitor cells of the embryonic rat neocortex and analyzed dendrite patterning three weeks later. Neurons overexpressing NOS1AP-L in layers II/III of the neocortex exhibit a reduction in dendrite length and number. Finally, to investigate the role that NOS1AP plays in human dendritic arbor development, human neurons were generated using induced pluripotent stem cell technology. Overexpression of either NOS1AP-L or NOS1AP-S in human neurons results in a decrease in dendrite branching. Interestingly, treatment of human neurons with D-serine results in a reduction in NOS1AP-L protein expression. Taken together, our data support a role for NOS1AP-L and NOS1AP-S in dendritogenesis and synaptic function.
NotePh.D.
NoteIncludes bibliographical references
Noteby Kristina Hernandez
Genretheses, ETD doctoral
Languageeng
CollectionGraduate School - New Brunswick Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.