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Creation of a novel ETV6-Cre reporter mouse model for ETV6-related fusion protein analysis and characterization of Ink4ab/ Vav-Cre transgenic mice Lines

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TitleInfo
Title
Creation of a novel ETV6-Cre reporter mouse model for ETV6-related fusion protein analysis and characterization of Ink4ab/ Vav-Cre transgenic mice Lines
Name (type = personal)
NamePart (type = family)
Jones
NamePart (type = given)
Daniel William
NamePart (type = date)
1987-
DisplayForm
Daniel William Jones
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Xie
NamePart (type = given)
Ping
DisplayForm
Ping Xie
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Padgett
NamePart (type = given)
Richard W.
DisplayForm
Richard W. Padgett
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Sabaawy
NamePart (type = given)
Hatem E.
DisplayForm
Hatem E. Sabaawy
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (encoding = w3cdtf); (qualifier = exact)
2015
DateOther (qualifier = exact); (type = degree)
2015-10
CopyrightDate (encoding = w3cdtf); (qualifier = exact)
2015
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Creation of a Novel ETV6-Cre Reporter Mouse Model for ETV6-Related Fusion Protein Analysis: The ETV6 gene is one of the most commonly targeted genes for chromosomal translocation in leukemia. ETV6 has been found to play a key role in hematopoiesis and maintenance of embryonic vascular development, yet its role in B-cell precursor acute lymphoblastic leukemia has yet to be fully determined in respect to both gene expression localization and interactions with other proteins. In this study, we focus on the creation of a novel transgenic line of ETV6-mCherry-CreERT2 mice that can be used both as a reporter for ETV6 expression and to drive ETV6-specific expression of Cre-ERT2 in mice with ETV6-related fusions using recombineering strategies. Characterization of Ink4ab/ Vav-Cre Transgenic Mice Lines: The Ink4ab genetic locus encodes for three essential proteins involved in tumor suppression, designated p15, p16, and p19 in mice. Full knockout models of this locus have been shown to exhibit accelerated tumorigenesis in multiple forms of cancer (Krimpenfort et al., 2007). By combining the Vav promoter, a promoter that first targets fetal liver hematopoietic stem cells, with a Cre-Lox system to allow full knock out of Ink4ab in only hematopoietic stem cells, we theorize the possibility of creating a hematopoietic system-based malignancy. Dissections have shown that Ink4ab+/-; Vav-Cre+ and Ink4ab-/-; Vav-Cre+ mice have splenic hyperplasia, tumor growth in both liver and spleen, and ascites in the peritoneal cavity. Peripheral blood analysis of Ink4ab-/-; Vav-Cre+ mice showed the presence of lymphoblasts, abnormal erythrocytes, and apoptotic neutrophils. Immunohistochemical staining has shown that Ink4ab-/-; Vav-Cre+ mice have increased apoptosis in T-cells, decreased mature B-cell and T-cell presence, increased cellular proliferation, deregulated cell cycle, and leukemia and lymphoma formation. These studies reveal a novel model for studying hematopoietic cell development, leukemia formation, and lymphoma formation.
Subject (authority = RUETD)
Topic
Cell and Developmental Biology
Subject (authority = ETD-LCSH)
Topic
Chromosomes
Subject (authority = ETD-LCSH)
Topic
Leukemia
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_6676
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (xiii, 65 p. : ill.)
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Daniel William Jones
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T35Q4Z2W
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Jones
GivenName
Daniel
MiddleName
William
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2015-08-27 15:51:28
AssociatedEntity
Name
Daniel Jones
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2015-10-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2017-10-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after October 30th, 2017.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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