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Activation of Grm1 expression by inducible mutated B-RAF in vitro and in vivo

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TitleInfo
Title
Activation of Grm1 expression by inducible mutated B-RAF in vitro and in vivo
Name (type = personal)
NamePart (type = family)
Sierra
NamePart (type = given)
Jairo L.
NamePart (type = date)
1987-
DisplayForm
Jairo L. Sierra
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Chen
NamePart (type = given)
Suzie
DisplayForm
Suzie Chen
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Goydos
NamePart (type = given)
James
DisplayForm
James Goydos
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Firestein
NamePart (type = given)
Bonnie
DisplayForm
Bonnie Firestein
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Merghoub
NamePart (type = given)
Taha
DisplayForm
Taha Merghoub
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
outside member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (encoding = w3cdtf); (qualifier = exact)
2015
DateOther (qualifier = exact); (type = degree)
2015-10
CopyrightDate (encoding = w3cdtf); (qualifier = exact)
2015
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Melanoma is a form of skin cancer that arises from the neoplastic transformation of melanocytes. Constitutive activation of the MAPK pathway due to mutations in the serine-threonine protein kinase B-RAF or N-RAS has been reported to play important roles in melanomagenesis. Previously, our laboratory identified that ectopic expression of metabotropic glutamate receptor 1 (Grm1) in melanocytes was sufficient to induce spontaneous melanoma development in transgenic mouse models (TG-3 and EPv). We also demonstrated that Grm1 expression activates the MAPK pathway. Recently, we detected Grm1 expression by immunohistochemical staining of tumors derived from transgenic mice harboring a B-RAF (V600E) mutation on PTEN null background. Similarly, Grm1 expression was also detected in tumor samples derived from transgenic mice with an N-RAS (Q61R) mutation on INK4a null background. In addition, several stable immortalized mouse melanocytic clones with exogenous mutant B-RAF (V600E) cDNA showed Grm1 expression in vitro. Since normal mouse melanocytes acquire loss of p16INK4a in order to become immortalized, we investigate whether loss of PTEN or INK4a/ARF was required for Grm1 expression by mutated B-RAF (V600E). We made use of a transgenic mouse model (BJB), which has an inducible B-RAF (V600E) genotype only in melanocytes. Here we show that induction of B-RAF (V600E) stimulates skin hyperpigmentation, presumably from increased proliferation of melanocytes. Results showed detectable Grm1 expression in BJB skin samples, suggesting that the induction of mutated B-RAF (V600E) may be sufficient to promote activation of Grm1 expression, and that ablation of PTEN expression/function but not of INK4a may be required.
Subject (authority = RUETD)
Topic
Cell and Developmental Biology
Subject (authority = ETD-LCSH)
Topic
Melanoma
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_6545
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (x, 57 p. : ill.)
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Jairo L. Sierra
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T36W9D26
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Sierra
GivenName
Jairo
MiddleName
L.
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2015-05-07 16:25:31
AssociatedEntity
Name
Jairo Sierra
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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