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Assessing life history tradeoffs in host range mutants of RNA bacteriophage phi6
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Watson, Aubrey Miles. Assessing life history tradeoffs in host range mutants of RNA bacteriophage phi6. Retrieved from https://doi.org/doi:10.7282/T33T9K6F

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TitleAssessing life history tradeoffs in host range mutants of RNA bacteriophage phi6
NameWatson, Aubrey Miles (author); Duffy, Siobain (chair); Bidle, Kay (internal member); Barkay, Tamar (internal member); Rutgers University; Graduate School - New Brunswick
Date Created2015
Other Date2015-10 (degree)
SubjectMicrobial Biology, Bacteriophages
Extent1 online resource (viii, 36 p. : ill.)
DescriptionStudies on host range mutation and its effects on phage fitness are common for bacteriophage phi6. Expanding to a novel host can occur through a large number of mutations which provide a new resource for use typically at a decreased level of reproduction. Secondary mutations developed during evolution on the novel host can raise phage fitness but may also lead to specialization on that host. Again, the mutations that are beneficial on the novel host are usually detrimental on the initial host. Studying phage life history traits such as fecundity (reproduction rate and size) as well as competitive ability on the hosts may help determine where fitness is lost. Here I used host range mutants of phi6 that were previously evolved on P. pseudoalcaligenes East River Isolate A (ERA). I performed paired growth assays on original host P. syringae pathovar phaseolicola (PP) and burst assays on both PP and ERA to determine the effects of these mutations on the phage’s reproductive ability. My selection of phages includes a specialist on PP, a specialist on ERA, and generalists capable of infecting more than one host. Paired growth assays showed that two of the five mutants suffer a fitness cost relative to the wild type ancestor on PP. However, burst assays showed no significant difference between the wild type and evolved mutants on PP. Burst assays on ERA showed that several mutants improved their burst time and all mutants improved their burst sizes compared to their ancestor on that host. The evolved mutants were not able to separate themselves from one another. Mutants separated by one mutation showed significant effects from these differences. My results suggest that burst and fitness assays can be a component for assessing differences in phage life history but need to be supplemented with further experimentation.
NoteM.S.
NoteIncludes bibliographical references
Noteby Aubrey Miles Watson
Genretheses, ETD graduate
Persistent URLhttps://doi.org/doi:10.7282/T33T9K6F
Languageeng
CollectionGraduate School - New Brunswick Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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