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Assessing life history tradeoffs in host range mutants of RNA bacteriophage phi6

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TitleInfo
Title
Assessing life history tradeoffs in host range mutants of RNA bacteriophage phi6
Name (type = personal)
NamePart (type = family)
Watson
NamePart (type = given)
Aubrey Miles
NamePart (type = date)
1991-
DisplayForm
Aubrey Miles Watson
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Duffy
NamePart (type = given)
Siobain
DisplayForm
Siobain Duffy
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Bidle
NamePart (type = given)
Kay
DisplayForm
Kay Bidle
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Barkay
NamePart (type = given)
Tamar
DisplayForm
Tamar Barkay
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (encoding = w3cdtf); (qualifier = exact)
2015
DateOther (qualifier = exact); (type = degree)
2015-10
CopyrightDate (encoding = w3cdtf); (qualifier = exact)
2015
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Studies on host range mutation and its effects on phage fitness are common for bacteriophage phi6. Expanding to a novel host can occur through a large number of mutations which provide a new resource for use typically at a decreased level of reproduction. Secondary mutations developed during evolution on the novel host can raise phage fitness but may also lead to specialization on that host. Again, the mutations that are beneficial on the novel host are usually detrimental on the initial host. Studying phage life history traits such as fecundity (reproduction rate and size) as well as competitive ability on the hosts may help determine where fitness is lost. Here I used host range mutants of phi6 that were previously evolved on P. pseudoalcaligenes East River Isolate A (ERA). I performed paired growth assays on original host P. syringae pathovar phaseolicola (PP) and burst assays on both PP and ERA to determine the effects of these mutations on the phage’s reproductive ability. My selection of phages includes a specialist on PP, a specialist on ERA, and generalists capable of infecting more than one host. Paired growth assays showed that two of the five mutants suffer a fitness cost relative to the wild type ancestor on PP. However, burst assays showed no significant difference between the wild type and evolved mutants on PP. Burst assays on ERA showed that several mutants improved their burst time and all mutants improved their burst sizes compared to their ancestor on that host. The evolved mutants were not able to separate themselves from one another. Mutants separated by one mutation showed significant effects from these differences. My results suggest that burst and fitness assays can be a component for assessing differences in phage life history but need to be supplemented with further experimentation.
Subject (authority = RUETD)
Topic
Microbial Biology
Subject (authority = ETD-LCSH)
Topic
Bacteriophages
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_6682
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (viii, 36 p. : ill.)
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Aubrey Miles Watson
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T33T9K6F
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Watson
GivenName
Aubrey
MiddleName
Miles
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2015-08-28 15:25:29
AssociatedEntity
Name
Aubrey Watson
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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