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Regulation of planar cell polarity by the Dachsous-Fat pathway

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TitleInfo
Title
Regulation of planar cell polarity by the Dachsous-Fat pathway
Name (type = personal)
NamePart (type = family)
Ambegaonkar
NamePart (type = given)
Abhijit Ashok
NamePart (type = date)
1985-
DisplayForm
Abhijit Ashok Ambegaonkar
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Grant
NamePart (type = given)
Barth
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Barth Grant
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Irvine
NamePart (type = given)
Kenneth
DisplayForm
Kenneth Irvine
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Steward
NamePart (type = given)
Ruth
DisplayForm
Ruth Steward
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Soto
NamePart (type = given)
Martha
DisplayForm
Martha Soto
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
outside member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (encoding = w3cdtf); (qualifier = exact)
2016
DateOther (qualifier = exact); (type = degree)
2016-01
CopyrightDate (encoding = w3cdtf); (qualifier = exact)
2015
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Planar cell polarity is the polarization of cells within the plane of a tissue. The Dachsous-Fat pathway plays a key role in the regulation of planar cell polarity and growth during the development of an organism. The transmembrane cadherins Fat and Dachsous from neighboring cells interact heterophilically and regulate the localization of the unconventional myosin Dachs in a cell. Four-jointed, a Golgi-localized kinase modulates the binding between Dachsous and Fat. Dachsous and Four-jointed are expressed in tissue-wide gradients, which influence Fat activity and direct the polarized membrane localization of Dachs. We demonstrate that differential expression of Dachsous or Four-jointed can modulate Dachs polarization at a distance in wing discs. This indicates that Dachsous and Four-jointed gradients can be measured over long range in a tissue through propagation of polarity. We also show that Dachsous and Fat are partially polarized along the endogenous Dachsous and Four-jointed gradients, providing a mechanism for propagation of polarity. Through directed membrane targeting of Dachs, we show that membrane localization of Dachs influences both planar cell polarity and the Hippo signaling pathway. These studies help in understanding the mechanisms involved in establishment and maintenance of planar cell polarity. The Frizzled pathway is another key pathway that regulates planar cell polarity, but its relationship with the Dachsous-Fat pathway was unclear. We demonstrate that Dachs and Dachsous can independently interact with a Frizzled pathway component, Spiny-legs, and direct its localization in vivo. Thus, the Dachsous-Fat pathway provides directional input to the Frizzled pathway by influencing the localization of Spiny-legs. These studies help in understanding how planar cell polarity is regulated in various tissues through coordination between Dachsous-Fat and Frizzled pathways. These studies also reveal that Spiny-legs and its isoform Prickle can respond to distinct planar cell polarity signals and allow the cells to compare and choose between these competing signals to direct polarity robustly in one direction. Thus, our results identify a mechanism for propagation of planar cell polarity through the tissue, establish the significance of Dachs membrane localization in Dachsous-Fat signaling and identify a molecular mechanism for crosstalk between the two planar cell polarity pathways.
Subject (authority = RUETD)
Topic
Cell and Developmental Biology
Subject (authority = ETD-LCSH)
Topic
Polarity (Biology)
Subject (authority = ETD-LCSH)
Topic
Cell differentiation
Subject (authority = ETD-LCSH)
Topic
Drosophia
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_6870
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (xv, 153 p. : ill.)
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Abhijit Ashok Ambegaonkar
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3R78H9M
Genre (authority = ExL-Esploro)
ETD doctoral
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Ambegaonkar
GivenName
Abhijit
MiddleName
Ashok
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2015-10-22 11:59:48
AssociatedEntity
Name
Abhijit Ambegaonkar
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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