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Regulation of meiotic central spindle function, homolog bi-orientation and co-orientation in Drosophila oocytes

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Title
Regulation of meiotic central spindle function, homolog bi-orientation and co-orientation in Drosophila oocytes
Name (type = personal)
NamePart (type = family)
Das
NamePart (type = given)
Arunika
NamePart (type = date)
1986-
DisplayForm
Arunika Das
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
McKim
NamePart (type = given)
Kim S.
DisplayForm
Kim S. McKim
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Barr
NamePart (type = given)
Maureen
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Maureen Barr
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Padgett
NamePart (type = given)
Richard
DisplayForm
Richard Padgett
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Kramer
NamePart (type = given)
Sunita
DisplayForm
Sunita Kramer
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
outside member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (encoding = w3cdtf); (qualifier = exact)
2016
DateOther (qualifier = exact); (type = degree)
2016-01
CopyrightDate (encoding = w3cdtf); (qualifier = exact)
2016
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
In the oocytes of many animals including humans, spindles are assembled in the absence of centrosomes. It is poorly understood what organizes the bipolar spindle in this system and directs attachment of chromosomes to opposing poles, a process known as bi-orientation. Previously it was shown that the kinesin 6 motor protein, Subito, is important for meiotic central spindle assembly and localization of the chromosomal passenger complex (CPC). I analyzed the role of different domains of Subito, to understand what restricts the activity of the motor to the chromatin, in meiosis. I found that the N-terminus of Subito, is required for localization of the kinesin to microtubules possibly in conjunction with the C-terminus. I also identified domains of Subito N-terminus that are required for mitosis, but may be dispensable for meiosis. I also analyzed mutants obtained from a synthetic lethal screen with Subito and found that the centralspindlin complex, is required for both homolog bi-orientation and Subito localization in meiosis. Surprisingly, I also found that downstream targets of this complex like Rho1 and Sticky (Citron kinase), are important for bi-orientation. In addition, another target from the screen, Polo kinase, which is a kinetochore protein is required for maintaining karyosome structure. Altogether, these results have given rise to a model where late cytokinesis proteins are involved in regulating central spindle assembly and directing bi-orientation possibly by modulating error correction by the kinase, Aurora B. It is unclear how the CPC function is regulated to establish tension between homologs and correct errors. Phosphatases have been shown to be important for a similar function in mitosis. However the role of phosphatases in meiosis was unknown. I have found that Drosophila PP1 is required for maintaining karyosome structure, peri-centromeric cohesion and co-orientation at metaphase I, in an Aurora B dependent manner. Kinetochore assembly by Aurora B, is also opposed by PP1. These results taken together provide insight on how Aurora B activity is balanced in meiosis, and emphasize the important role played by PP1 in oocytes.
Subject (authority = RUETD)
Topic
Cell and Developmental Biology
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_6915
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (xv, 163 p. : ill.)
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Subject (authority = ETD-LCSH)
Topic
Drosophila
Subject (authority = ETD-LCSH)
Topic
Meiosis
Note (type = statement of responsibility)
by Arunika Das
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
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NjNbRU
Identifier (type = doi)
doi:10.7282/T34F1SS1
Genre (authority = ExL-Esploro)
ETD doctoral
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Das
GivenName
Arunika
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2015-12-15 16:00:41
AssociatedEntity
Name
Arunika Das
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2016-01-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2018-01-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after January 30th, 2018.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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