Inflammation is a complicated physiological and pathological process in response to stimuli. In general, a controlled inflammatory response is a beneficial defense system, which provides protection against infection. However, it can become detrimental when the response is prolonged and dysregulated called chronic inflammation. Over the past several years, chronic inflammation has been recognized to be the root of various human diseases, including neurological disorders, metabolic disorders, obesity, cardiovascular diseases and cancers. Resveratrol is a well-known natural antioxidative and anti-inflammatory compound from grapes. In the previous study, the antioxidative properties of resveratrol metabolites have been suggested to be more active than resveratrol. δ-Viniferin is a main resveratrol dehydrodimer metabolite and has been identified in grape cell cultures and wines. It possesses two resorcinol skeletons, which are determinants of radical scavenging and antioxidative potential. Hence, we researched in the anti-inflammatory effect of δ-viniferin. We prepared this compound in vitro by the oxidative dimerization of resveratrol with horseradish peroxidase in the presence of H2O2. In this study, the cytotoxic and anti-inflammatory effects on LPS-stimulated murine macrophage were examined. According to the data, we found that δ-viniferin suppressed the levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and phosphorylated inhibitor κBα (p-IκBα) protein expressions, as well as the down-stream product, nitric oxide, in murine RAW 264.7 cells induced with lipopolysaccharide (LPS). Moreover, the inhibitory effects of δ-viniferin on inflammation associated signaling pathways, such as the blockade of LPS-induced IκBα phosphorylation and degradation, the decrease of PI3K and Akt phosphorylation, and the reduction of LPS-induced transcriptional activity of NF-κB. According to the results, δ-viniferin might have potential to be developed into an effective anti-inflammatory agent.
Subject (authority = RUETD)
Topic
Food Science
Subject (authority = ETD-LCSH)
Topic
Anti-inflammatory agents
Subject (authority = ETD-LCSH)
Topic
Resveratrol
Subject (authority = ETD-LCSH)
Topic
Inflammation
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_6982
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (x, 56 p. : ill.)
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Pei-Hsuan Hsieh
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.