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Characterization of three distinct arsenic resistant microorganisms isolated from the agricultural soils of Mekong Delta in Vietnam
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Jain, Ishita. Characterization of three distinct arsenic resistant microorganisms isolated from the agricultural soils of Mekong Delta in Vietnam. Retrieved from https://doi.org/doi:10.7282/T3251M8V

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TitleCharacterization of three distinct arsenic resistant microorganisms isolated from the agricultural soils of Mekong Delta in Vietnam
NameJain, Ishita (author); Häggblom, Max M. (chair); Rutgers University; Graduate School - New Brunswick
Date Created2016
Other Date2016-01 (degree)
SubjectMicrobial Biology, Mekong River Delta (Vietnam and Cambodia), Microorganisms, Arsenic
Extent1 online resource (ix, 88 p. : ill.)
DescriptionArsenic is a naturally occurring metalloid in the earth’s crust. Microbial processes play an evident and vital role in biogeochemical cycling of elements. Arsenic and its forms vary in toxicity and mobility, and its contamination can be a major problem for the living beings and the environment. The Mekong River delta region in Vietnam is a populated area and the fertile fields are used for cultivating rice, wheat and maize. Arsenic accumulation in the agricultural soils and groundwater leads to exposure by direct contact or by being absorbed and accumulated in the food crops. Microorganisms have developed the ability to either utilize arsenic species for metabolism or for bioconversion to reduce its lethality, which affects arsenic mobility, bioavailability in the environment. This study aims to isolate and identify microorganisms in these contaminated soil samples by enrichment culture technique, T-RFLP fingerprinting, genetic analysis through PCR amplification, and HPLC method to monitor arsenate reduction to arsenite. Three distinct arsenate reducing strains were isolated from the rice and maize soil samples and they belong to the genera Shewanella, Klebsiella and Enterobacter, respectively. Members of these genera are known to transform arsenic species. Arsenic methylation is being explored by researchers as the methylated forms can be absorbed by food crops, especially by the rice plants. Rice is a staple food crop and high arsenic concentrations in the grain will lead to exposure and bioaccumulation when ingested. The three arsentate-reducing bacterial strains isolated in this study may also possibly methylate arsenite, as methylated forms were detected through HPLC and the presence of the requisite genese by PCR experiments. This work contributes to the current knowledge of arsenic biotransformation by microorganisms, specifically in the Mekong River delta, and this information can then be useful in mitigating arsenic contamination.
NoteM.S.
NoteIncludes bibliographical references
Noteby Ishita Jain
Genretheses, ETD graduate
Persistent URLhttps://doi.org/doi:10.7282/T3251M8V
Languageeng
CollectionGraduate School - New Brunswick Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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