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Characterization of three distinct arsenic resistant microorganisms isolated from the agricultural soils of Mekong Delta in Vietnam
Descriptive
TitleInfo
Title
Characterization of three distinct arsenic resistant microorganisms isolated from the agricultural soils of Mekong Delta in Vietnam
Name
(type = personal)
NamePart
(type = family)
Jain
NamePart
(type = given)
Ishita
NamePart
(type = date)
1991-
DisplayForm
Ishita Jain
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Häggblom
NamePart
(type = given)
Max M.
DisplayForm
Max M. Häggblom
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
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(qualifier = exact)
2016
DateOther
(qualifier = exact);
(type = degree)
2016-01
CopyrightDate
(encoding = w3cdtf);
(qualifier = exact)
2016
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Arsenic is a naturally occurring metalloid in the earth’s crust. Microbial processes play an evident and vital role in biogeochemical cycling of elements. Arsenic and its forms vary in toxicity and mobility, and its contamination can be a major problem for the living beings and the environment. The Mekong River delta region in Vietnam is a populated area and the fertile fields are used for cultivating rice, wheat and maize. Arsenic accumulation in the agricultural soils and groundwater leads to exposure by direct contact or by being absorbed and accumulated in the food crops. Microorganisms have developed the ability to either utilize arsenic species for metabolism or for bioconversion to reduce its lethality, which affects arsenic mobility, bioavailability in the environment. This study aims to isolate and identify microorganisms in these contaminated soil samples by enrichment culture technique, T-RFLP fingerprinting, genetic analysis through PCR amplification, and HPLC method to monitor arsenate reduction to arsenite. Three distinct arsenate reducing strains were isolated from the rice and maize soil samples and they belong to the genera Shewanella, Klebsiella and Enterobacter, respectively. Members of these genera are known to transform arsenic species. Arsenic methylation is being explored by researchers as the methylated forms can be absorbed by food crops, especially by the rice plants. Rice is a staple food crop and high arsenic concentrations in the grain will lead to exposure and bioaccumulation when ingested. The three arsentate-reducing bacterial strains isolated in this study may also possibly methylate arsenite, as methylated forms were detected through HPLC and the presence of the requisite genese by PCR experiments. This work contributes to the current knowledge of arsenic biotransformation by microorganisms, specifically in the Mekong River delta, and this information can then be useful in mitigating arsenic contamination.
Subject
(authority = RUETD)
Topic
Microbial Biology
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_7015
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (ix, 88 p. : ill.)
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
Subject
(authority = ETD-LCSH)
Topic
Mekong River Delta (Vietnam and Cambodia)
Subject
(authority = ETD-LCSH)
Topic
Microorganisms
Subject
(authority = ETD-LCSH)
Topic
Arsenic
Note
(type = statement of responsibility)
by Ishita Jain
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3251M8V
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Jain
GivenName
Ishita
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
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(qualifier = exact);
(point = start)
2016-01-13 12:38:51
AssociatedEntity
Name
Ishita Jain
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
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(qualifier = exact);
(point = start)
2016-01-31
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = end)
2018-01-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after January 30th, 2018.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
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ContentModel
ETD
OperatingSystem
(VERSION = 5.1)
windows xp
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Statistical Profile