Jespersen, David. Metabolic and molecular factors associated with heat-induced leaf senescence in Agrostis (ssp.). Retrieved from https://doi.org/doi:10.7282/T3XD13R3
DescriptionHeat stress is a major abiotic stress affecting many plants world wide. Bentgrasses (Agrostis ssp.) are a genus which contains several cool-season perennial grass species which are used on high value turf areas such as golf course green. During summer months when temperatures are elevated bentgrasses frequently experience heat-induced leaf senescence characterized by alterations to metabolism and degradation of cellular constituents including membranes, pigments and proteins. Better understanding of physiological and molecular processes which affect heat-induced leaf senescence will aid in the developments of more heat tolerant bentgrasses. Research goals were to look at physiological, biochemical, molecular genetic difference during heat stress events play roles in delaying heat-induced senescence. This was accomplished in several studies which included a comparison in physiological responses of multiple cultivars exposed to heat stress; proteomic analysis of membrane proteins affected by heat stress as membranes are one of the first sites of heat damages; analysis of the effects of several compounds include cytokinin, an ethylene inhibitor, and nitrogen and how they delay heat-induced senescence and their effects on the plants proteome and metabolome; quantitative trait loci analysis to identify genetic regions associated with important heat tolerance traits; development of candidate gene markers associated with important metabolic functions related to heat-induced senescence and their association with physiological traits related to heat tolerance; investigation of chlorophyll biosynthesis and degradation pathways to help elucidate the cause of chlorophyll loss from leaves during heat-induced senescence. The integration of this information will not only expand our knowledge of heat-induced senescence in cool season turfgrasses but may be used for marker-assisted selection to develop improved cultivars with delayed stress-induced senescence and maintain plant health and growth during heat stress events.