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Studies on the effect of cell cycle arrest on central metabolism in the diatom Phaeodactylum tricornutum, using physiological and systems biology approaches
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Kim, Joomi. Studies on the effect of cell cycle arrest on central metabolism in the diatom Phaeodactylum tricornutum, using physiological and systems biology approaches. Retrieved from https://doi.org/doi:10.7282/T34X59VG

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TitleStudies on the effect of cell cycle arrest on central metabolism in the diatom Phaeodactylum tricornutum, using physiological and systems biology approaches
NameKim, Joomi (author); Falkowski, Paul G. (chair); Bhattacharya, Debashish (internal member); Lun, Desmond S. (internal member); Bidle, Kay D. (internal member); Falciatore, Angela (outside member); Rutgers University; Graduate School - New Brunswick
Date Created2016
Other Date2016-01 (degree)
SubjectOceanography, Diatoms
Extent1 online resource (x, 142 p. : ill.)
DescriptionDiatoms (Bacillarophyceae) are photosynthetic unicellular microalgae that have risen to ecological prominence in the modern oceans over the past 30 million years. They are excellent candidates for biodiesel feedstocks. Global climate change has led to an interest in algal triacylglycerols (TAGs) as feedstocks for sustainable biodiesel, and diatoms are attractive candidates for TAG production as one of the most productive and environmentally flexible algae in the contemporary oceans. For Chapter 2, a genome-scale metabolic model was constructed to calculate intracellular fluxes of a diatom under different growth conditions. The model identified enzymes that may be relevant to increasing lipid synthesis, explored how transporters affect flux outputs, and explored unusual features of diatoms, including the Entner-Douderoff and phosphoketolase pathways, and glycolytic enzymes in their mitochondria. Chapter 3 discusses how cell cycle arrest via cyclin-dependent kinase (Cdk) inhibition, can increase accumulation of TAGs, and shift metabolism away from protein synthesis. For Chapter 4, transcriptome analysis of cells under cell cycle arrest was performed to show that the pattern of gene expression was fundamentally different from nitrogen stress. Most of the genes related to fatty acid and TAG synthesis were up-regulated. The gene expression pattern for light harvesting complexes was similar to cells stressed by high light, suggesting that arrested cells have smaller sinks for photosynthetically generated electrons.
NotePh.D.
NoteIncludes bibliographical references
Noteby Joomi Kim
Genretheses, ETD doctoral
Persistent URLhttps://doi.org/doi:10.7282/T34X59VG
Languageeng
CollectionGraduate School - New Brunswick Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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