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Growth inhibitory effects of 3'-hydroxypterostilbene in human prostate cancer cells and xenograft mice
Descriptive
TitleInfo
Title
Growth inhibitory effects of 3'-hydroxypterostilbene in human prostate cancer cells and xenograft mice
Name
(type = personal)
NamePart
(type = family)
Tsai
NamePart
(type = given)
Hui-Yun
NamePart
(type = date)
1983-
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Hui-Yun Tsai
Role
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(authority = RULIB)
author
Name
(type = personal)
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(type = family)
HO
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(type = given)
CHI-TANG
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CHI-TANG HO
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Advisory Committee
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chair
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Lee
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Tung-Ching
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Tung-Ching Lee
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Advisory Committee
Role
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internal member
Name
(type = personal)
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(type = family)
HUANG
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(type = given)
QINGRONG
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QINGRONG HUANG
Affiliation
Advisory Committee
Role
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(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Wu
NamePart
(type = given)
Qing-Li
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Qing-Li Wu
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Li
NamePart
(type = given)
Shiming
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Shiming Li
Affiliation
Advisory Committee
Role
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(authority = RULIB)
outside member
Name
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NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
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NamePart
Graduate School - New Brunswick
Role
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(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
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(qualifier = exact)
2016
DateOther
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(type = degree)
2016-01
CopyrightDate
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(qualifier = exact)
2016
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Prostate cancer is the second highest cause of cancer-related death among men in the United States. Most of these deaths are caused by invasive and metastatic spread of the prostate cancer. Therefore, more efforts should be dedicated to the development of preventive strategies to reduce prostate cancer prevalence and impact. Pterostilbene (Pt) is a natural antioxidant compound predominantly found in blueberries, grapes and a tree wood, Pterocarpus marsupium. 3'-Hydroxypterostilbene (OHPt), one of hydroxyl analogs of pterostilbene, can be isolated from whole plant of the herb Sphaerophysa salsula, a shrub widely distributed in central Asia and northwest China. The objective of this study was to investigate the growth inhibitory effects of 3'-hydroxypterostilbene on human prostate cancer cells in vitro and in vivo. The results showed that OHPt significantly decreased cell viability of PC-3 and LNCaP cells in a dose- and time-dependent manner. However, OHPt showed much stronger inhibitory effects on the growth of the prostate cancer cells in comparison with its parent compound Pt, suggesting that addition of an OH group to the 3’-position of Pt ameliorated its anti-cancer cell proliferative activity. Next, we further elucidated the molecular mechanisms of anti-proliferation of human prostate cancer cells by OHPt. Treatment with OHPt induced apoptosis via disruption of mitochondrial membrane integrity and activation of caspase-8, caspase-9 and caspase-3 in both PC-3 and LNCaP cells. Moreover, OHPt increased the ratio of proapototic protein Bax to anti-apoptotic protein Bcl-xL. These findings suggested that OHPt had a strong growth inhibitory effect on PC-3 and LNCaP cells through induction of intrinsic and extrinsic apoptotic pathway. Besides, OHPt induced autophagic cell death by upregulating the expression of Beclin-1, LC3 proteins and autophagosome formation. Furthermore, in vivo study, OHPt showed potent anti-tumor effects in PC-3 xenograft nude mice. Treatment of mice with OHPt efficiently inhibited the tumor volume and tumor weight. Taken together, the findings of this research provide insights that OHPt might have significant chemotherapeutic applications for the future management of prostate cancer.
Subject
(authority = RUETD)
Topic
Food Science
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_6978
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (xv, 120 p. : ill.)
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Subject
(authority = ETD-LCSH)
Topic
Prostate--Cancer--Prevention
Subject
(authority = ETD-LCSH)
Topic
Antioxidants
Note
(type = statement of responsibility)
by Hui-Yun Tsai
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T30R9RGG
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Tsai
GivenName
Hui-Yun
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2016-01-07 17:04:25
AssociatedEntity
Name
Hui-Yun Tsai
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
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(qualifier = exact);
(point = start)
2016-01-31
DateTime
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(qualifier = exact);
(point = end)
2018-01-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after January 30th, 2018.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
(ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem
(VERSION = 5.1)
windows xp
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