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Macrosteatotic hepatocyte defatting

Descriptive

TitleInfo
Title
Macrosteatotic hepatocyte defatting
SubTitle
optimization through reaction-flow modeling
Name (type = personal)
NamePart (type = family)
Yarmush
NamePart (type = given)
Gabriel
NamePart (type = date)
1984-
DisplayForm
Gabriel Yarmush
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Berthiaume
NamePart (type = given)
Francois
DisplayForm
Francois Berthiaume
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Maguire
NamePart (type = given)
Tim
DisplayForm
Tim Maguire
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Schloss
NamePart (type = given)
Rene
DisplayForm
Rene Schloss
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Guarrera
NamePart (type = given)
James
DisplayForm
James Guarrera
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
outside member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (encoding = w3cdtf); (qualifier = exact)
2016
DateOther (qualifier = exact); (type = degree)
2016-01
CopyrightDate (encoding = w3cdtf); (qualifier = exact)
2016
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Since there is a shortage of transplantable livers, the donor pool needs to be increased. One method to accomplish this is to recondition extended criteria donor grafts, a large portion of which are moderate to severe macrosteatotic livers. Transplantation of these livers often leads to primary nonfunction caused by an increased susceptibility to the effects of ischemia reperfusion injury (I/R) that result from the harvesting, transportation, and transplantation of the liver. Our lab has developed a novel procedure to recondition these livers through an ex vivo perfusion that will reduce the hepatic triglyceride content before the onset of the effects of I/R injury. To be feasibly performed in a clinical setting, the defatting must be completed in a matter of hours. While attempts to identify the ideal defatting cocktail in static culture only resulted in defatting livers after days of treatment, the rate of defatting increases to more a clinically relevant timeframe in a flow environment. My project focuses on understanding the differences between a static and flow environment and using this information to develop the ideal parameters for defatting. I hypothesize that using flow and appropriate defatting agents, steatotic hepatocytes can be defatted in a clinically relevant time of hours, without harming viability or function, to a level that reduces or eliminates increased sensitivity to I/R injury. In order to prove the hypothesis I will use the following 3 specific aims: 1. Develop a metabolic flux analysis model to explore defatting metabolic pathways. 2. Develop a computational fluid dynamics and kinetic model of a vitro flow reactor to optimize key transport parameters. 3. Validate the optimal conditions of flow has on the viability and function in hepatocyte cell lines.
Subject (authority = RUETD)
Topic
Biomedical Engineering
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_7006
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (viii, 52 p. : ill.)
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Subject (authority = ETD-LCSH)
Topic
Fatty degeneration
Subject (authority = ETD-LCSH)
Topic
Liver--Transplantation
Subject (authority = ETD-LCSH)
Topic
Liver cells
Note (type = statement of responsibility)
by Gabriel Yarmush
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T33J3G1X
Genre (authority = ExL-Esploro)
ETD doctoral
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Yarmush
GivenName
Gabriel
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2016-01-11 22:30:18
AssociatedEntity
Name
Gabriel Yarmush
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
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