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Identification of a Ruminococcaceae Species as the Methyl tert-Butyl Ether (MTBE) Degrading Bacterium in a Methanogenic Consortium

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TypeOfResource
Text
TitleInfo
Title
Identification of a Ruminococcaceae Species as the Methyl tert-Butyl Ether (MTBE) Degrading Bacterium in a Methanogenic Consortium
Name (type = personal)
NamePart (type = family)
Liu
NamePart (type = given)
Tong
Role
RoleTerm (authority = marcrt); (type = text)
author
Affiliation
Microbiology and Molecular Genetics (DMMG), Rutgers University
Name (type = personal)
NamePart (type = family)
Ahn
NamePart (type = given)
Hyeri
Role
RoleTerm (authority = marcrt); (type = text)
author
Affiliation
Microbiology and Molecular Genetics (DMMG), Rutgers University
Name (type = personal)
NamePart (type = family)
Sun
NamePart (type = given)
Weimin
Role
RoleTerm (authority = marcrt); (type = text)
author
Affiliation
Biochemistry and Microbiology, Rutgers University
Name (type = personal)
NamePart (type = family)
McGuinness
NamePart (type = given)
Lora R.
Role
RoleTerm (authority = marcrt); (type = text)
author
Affiliation
Marine and Coastal Sciences, Rutgers University
Name (type = personal)
NamePart (type = family)
Kerkhof
NamePart (type = given)
Lee J.
Role
RoleTerm (authority = marcrt); (type = text)
author
Affiliation
Marine and Coastal Sciences, Rutgers University
Name (authority = orcid); (authorityURI = http://id.loc.gov/vocabulary/identifiers/orcid.html); (type = personal); (valueURI = http://orcid.org/0000-0001-6307-7863)
NamePart (type = family)
Häggblom
NamePart (type = given)
Max M.
Affiliation
Biochemistry and Microbiology, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (authority = RutgersOrg-Department); (type = corporate)
NamePart
Biochemistry and Microbiology
Name (authority = RutgersOrg-School); (type = corporate)
NamePart
School of Environmental and Biological Sciences (SEBS)
Name (authority = RutgersOrg-Department); (type = corporate)
NamePart
Marine and Coastal Sciences
Name (authority = RutgersOrg-Department); (type = corporate)
NamePart
Microbiology and Molecular Genetics (DMMG)
Name (authority = RutgersOrg-School); (type = corporate)
NamePart
New Jersey Medical School (NJMS)
Genre (authority = RULIB-FS)
Article, Refereed
Genre (authority = NISO JAV)
Accepted Manuscript (AM)
OriginInfo
DateIssued (encoding = w3cdtf); (keyDate = yes); (qualifier = exact)
2016
Abstract (type = Abstract)
The widespread use of methyl tert-butyl ether (MTBE) has caused major contamination of groundwater sources and is a concern due to its taste and odor problems, as well as toxicity. MTBE can be degraded anaerobically which makes bioremediation of contaminated aquifers a potential solution. Nevertheless, the organisms and mechanisms that are responsible for anaerobic MTBE degradation are still unknown. The aim of our research was to identify the organisms actively degrading MTBE. For this purpose we characterized an anaerobic methanogenic culture enriched with MTBE as the sole carbon source from the New Jersey Arthur Kill intertidal strait sediment. The cultures were analyzed using stable isotope probing (SIP) combined with terminal restriction fragment length polymorphism (T-RFLP), high-throughput sequencing and clone library analysis of bacterial 16S rRNA genes. The sequence data indicated that phylotypes belonging to the Ruminococcaceae in the Firmicutes were predominant in the methanogenic cultures. SIP experiments also showed sequential incorporation of the 13C labeled MTBE by the bacterial community with a bacterium most closely related to Saccharofermentans acetigenes identified as the bacterium active in O-demethylation of MTBE. Identification of the microorganisms responsible for the activity will help us better understand anaerobic MTBE degradation processes in the field and determine biomarkers for monitoring natural attenuation.
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
PhysicalDescription
InternetMediaType
application/pdf
Extent
42 p.
Extension
DescriptiveEvent
Type
Citation
DateTime (encoding = w3cdtf)
2016
AssociatedObject
Name
Environmental Science & Technology
Type
Journal
Relationship
Has part
Detail
1455-1464
Identifier (type = volume and issue)
50(3)
Reference (type = url)
http://dx.doi.org/10.1021/acs.est.5b04731
Extension
DescriptiveEvent
Type
Grant award
AssociatedEntity
Role
Funder
Name
National Science Foundation
AssociatedEntity
Role
Originator
Name
Max M. Häggblom
AssociatedObject
Type
Grant number
Name
CEBT 1335824
Subject (authority = LCSH)
Topic
Butyl methyl ether
Subject (authority = local)
Topic
MTBE (Chemical)
Subject (authority = LCSH)
Topic
Butyl methyl ether--Biodegradation
Subject (authority = local)
Topic
Ruminococcaceae
Note (type = peerReview)
Peer reviewed
RelatedItem (type = host)
TitleInfo
Identifier (type = local)
rucore30202400001
RelatedItem (type = host)
TitleInfo
Title
Sun, Weimin
Identifier (type = local)
rucore30202600001
RelatedItem (type = host)
TitleInfo
Title
McGuinness, Lora R.
Identifier (type = local)
rucore30202700001
RelatedItem (type = host)
TitleInfo
Title
Kerkhof, Lee J.
Identifier (type = local)
rucore30202800001
RelatedItem (type = host)
TitleInfo
Title
Liu, Tong
Identifier (type = local)
rucore30202500001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3JS9SHN
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Rights

RightsDeclaration (AUTHORITY = FS); (ID = rulibRdec0004)
Copyright for scholarly resources published in RUcore is retained by the copyright holder. By virtue of its appearance in this open access medium, you are free to use this resource, with proper attribution, in educational and other non-commercial settings. Other uses, such as reproduction or republication, may require the permission of the copyright holder.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsEvent
Type
Permission or license
AssociatedObject
Type
License
Name
Multiple author license v. 1
Detail
I hereby grant to Rutgers, The State University of New Jersey (Rutgers) the non-exclusive right to retain, reproduce, and distribute the deposited work (Work) in whole or in part, in and from its electronic format, without fee. This agreement does not represent a transfer of copyright to Rutgers.Rutgers may make and keep more than one copy of the Work for purposes of security, backup, preservation, and access and may migrate the Work to any medium or format for the purpose of preservation and access in the future. Rutgers will not make any alteration, other than as allowed by this agreement, to the Work.I represent and warrant to Rutgers that the Work is my original work. I also represent that the Work does not, to the best of my knowledge, infringe or violate any rights of others.I further represent and warrant that I have obtained all necessary rights to permit Rutgers to reproduce and distribute the Work and that any third-party owned content is clearly identified and acknowledged within the Work.By granting this license, I acknowledge that I have read and agreed to the terms of this agreement and all related RUcore and Rutgers policies.
RightsEvent
Type
Publication notice
Detail
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Environmental Science & Technology, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.est.5b04731.
RightsEvent
Type
Embargo
DateTime (point = start); (encoding = w3cdtf)
2016-04-28
Detail
Access to this PDF has been restricted due to the policy of the publisher, ACS Publications, which requires a 12 month embargo from the date the article was published. The article in this repository will be publicly available after January 4, 2017.
DateTime (point = end); (encoding = w3cdtf)
2017-01-04
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ContentModel
Document
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