Staff View
Unilateral partial nephrectomy with warm ischemia results in acute Hypoxia inducible factor 1-alpha (HIF-1α) and toll-like receptor 4 (TLR4) overexpression in a porcine model

Descriptive

TypeOfResource
Text
TitleInfo
Title
Unilateral partial nephrectomy with warm ischemia results in acute Hypoxia inducible factor 1-alpha (HIF-1α) and toll-like receptor 4 (TLR4) overexpression in a porcine model
Name (type = personal)
NamePart (type = family)
Zhang
NamePart (type = given)
Zhiyong
Affiliation
Urology (Robert Wood Johnson Medical School, RWJMS), Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Haimovich
NamePart (type = given)
Beatrice
Affiliation
Surgery (Robert Wood Johnson Medical School, RWJMS), Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Kwon
NamePart (type = given)
Young Suk
Affiliation
Cancer Institute of New Jersey (CINJ), Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
Name (type = personal)
NamePart (type = family)
Lu
NamePart (type = given)
Tyler
Affiliation
Robert Wood Johnson Medical School (RWJMS), Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Fyfe-Kirschner
NamePart (type = given)
Billie
Role
RoleTerm (authority = marcrt); (type = text)
author
Affiliation
Pathology and Laboratory Medicine (Robert Wood Johnson Medical School, RWJMS), Rutgers University
Name (type = personal)
NamePart (type = family)
Olweny
NamePart (type = given)
Ephrem O.
Affiliation
Urology (Robert Wood Johnson Medical School, RWJMS), Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (authority = RutgersOrg-School); (type = corporate)
NamePart
Robert Wood Johnson Medical School (RWJMS)
Name (authority = RutgersOrg-Department); (type = corporate)
NamePart
Pathology and Laboratory Medicine (Robert Wood Johnson Medical School, RWJMS)
Name (authority = RutgersOrg-Department); (type = corporate)
NamePart
Surgery (Robert Wood Johnson Medical School, RWJMS)
Name (authority = RutgersOrg-Department); (type = corporate)
NamePart
Urology (Robert Wood Johnson Medical School, RWJMS)
Name (authority = RutgersOrg-School); (type = corporate)
NamePart
Cancer Institute of New Jersey (CINJ)
Genre (authority = RULIB-FS)
Article, Refereed
Genre (authority = NISO JAV)
Version of Record (VoR)
Note (type = peerReview)
Peer reviewed
OriginInfo
DateIssued (encoding = w3cdtf); (keyDate = yes); (qualifier = exact)
2016
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
PhysicalDescription
InternetMediaType
application/pdf
Extent
9 p.
Extension
DescriptiveEvent
Type
Grant award
AssociatedEntity
Role
Funder
Name
New Jersey Health Foundation Inc
AssociatedEntity
Role
Originator
Name
Ephrem O. Olweny
AssociatedObject
Type
Grant number
Name
508593
Extension
DescriptiveEvent
Type
Citation
DateTime (encoding = w3cdtf)
2016
AssociatedObject
Type
Journal
Relationship
Has part
Name
PLoS ONE
Identifier (type = volume and issue)
11(5)
Reference (type = url)
http://dx.doi.org/10.1371/journal.pone.0154708
Detail
1-7
Subject (authority = LCSH)
Topic
Kidneys--Wounds and injuries
Subject (authority = local)
Topic
Hypoxia inducible factor 1-alpha (HIF-1α)
Subject (authority = local)
Topic
Toll-like receptor 4 (TLR4)
Abstract (type = abstract)
Purpose: Ischemia/reperfusion (I/R) during partial nephrectomy (PN) contributes to acute kidney injury (AKI), which is inaccurately assessed using existent clinical markers of renal function. We evaluated I/R-related changes in expression in hypoxia inducible factor 1α (HIF-1α) and toll-like receptor 4 (TLR4), within kidney tissue and peripheral blood leukocytes (PBL) in a porcine model of PN.

Materials and Methods: Three adult pigs each underwent unilateral renal hilar cross clamping for 180 min followed by a 15 min reperfusion. The contralateral kidney served as control. Biopsies of clamped kidneys were obtained at baseline (time 0), every 60 min during the hypoxic phase, and post-reperfusion. Control kidneys were biopsied once at 180 min. Peripheral blood was sampled at time 0, every 30 min during the hypoxic phase, and post-reperfusion. HIF-1α and TLR4 expression in kidney tissue and PBL were analyzed by Western blotting. I/R-related histological changes were assessed.

Results: Expression of HIF-1α in clamped kidneys and PBL was below detection level at baseline, rising to detectable levels after 60 min of hypoxia, and continuing to rise throughout the hypoxic and reperfusion phases. Expression of TLR-4 in clamped kidneys followed a similar trend with initial detection after 30–60 min of hypoxia. Control kidneys exhibited no change in HIF-1α or TLR-4 expression. I/R-related histologic changes were minimal, primarily mild tubular dilatation.

Conclusions: In a porcine model of PN, HIF-1α and TLR4 exhibited robust, I/R-related increases in expression in kidney tissue and PBL. Further studies investigating these molecules as potential markers of AKI are warranted.
RelatedItem (type = host)
TitleInfo
Title
Olweny, Ephrem O.
Identifier (type = local)
rucore30204100001
RelatedItem (type = host)
TitleInfo
Title
Haimovich, Beatrice
Identifier (type = local)
rucore30205400001
RelatedItem (type = host)
TitleInfo
Title
Kwon, Young Suk
Identifier (type = local)
rucore30205500001
RelatedItem (type = host)
TitleInfo
Title
Fyfe-Kirschner, Billie
Identifier (type = local)
rucore30205700001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3PV6NGB
Genre (authority = ExL-Esploro)
Journal Article
Back to the top

Rights

RightsDeclaration (AUTHORITY = FS); (ID = rulibRdec0004); (TYPE = [FS] statement #1)
Copyright for scholarly resources published in RUcore is retained by the copyright holder. By virtue of its appearance in this open access medium, you are free to use this resource, with proper attribution, in educational and other non-commercial settings. Other uses, such as reproduction or republication, may require the permission of the copyright holder.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsEvent
Type
Permission or license
AssociatedObject
Type
License
Name
Multiple author license v. 1
Detail
I hereby grant to Rutgers, The State University of New Jersey (Rutgers) the non-exclusive right to retain, reproduce, and distribute the deposited work (Work) in whole or in part, in and from its electronic format, without fee. This agreement does not represent a transfer of copyright to Rutgers. Rutgers may make and keep more than one copy of the Work for purposes of security, backup, preservation, and access and may migrate the Work to any medium or format for the purpose of preservation and access in the future. Rutgers will not make any alteration, other than as allowed by this agreement, to the Work. I represent and warrant to Rutgers that the Work is my original work. I also represent that the Work does not, to the best of my knowledge, infringe or violate any rights of others. I further represent and warrant that I have obtained all necessary rights to permit Rutgers to reproduce and distribute the Work and that any third-party owned content is clearly identified and acknowledged within the Work. By granting this license, I acknowledge that I have read and agreed to the terms of this agreement and all related RUcore and Rutgers policies.
RightsHolder (type = personal)
Name
FamilyName
Zhang
GivenName
Zhiyong
Role
Copyright holder
RightsHolder (type = personal)
Name
FamilyName
Haimovich
GivenName
Beatrice
Role
Copyright holder
RightsHolder (type = personal)
Name
FamilyName
Kwon
GivenName
Young
MiddleName
Suk
Role
Copyright holder
RightsHolder (type = personal)
Name
FamilyName
Lu
GivenName
Tyler
Role
Copyright holder
RightsHolder (type = personal)
Name
FamilyName
Fyfe-Kirschner
GivenName
Billie
Role
Copyright holder
RightsHolder (type = personal)
Name
FamilyName
Olweny
GivenName
Ephrem
MiddleName
Odoy
Role
Copyright holder
Back to the top

Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
Document
Back to the top
Version 8.3.13
Rutgers University Libraries - Copyright ©2020