DescriptionMicrofluidic systems provide a distinct environment to study crystallization because of their unique properties, which include their superior heat and mass transport control. While continuous flow platforms are particularly appealing for crystal synthesis because of their relative simplicity, crystal adhesion to the channel wall is routinely a problematic issue. However, appropriately configured systems can minimize this problem. To this end, a non-droplet-based system that reduces or circumvents the problematic issue of crystal adhesion during crystallization in microfluidic devices has been developed. The capabilities of this system with respect to the effect of local system parameters (e.g., calcium and oxalate ion concentration, and the concentration of certain pertinent chemical species) on the calcium oxalate (CaOx) crystallization process have been examined. A variety of microscopy-based methods (including scanning electron, confocal Raman and Fourier Transform Infrared microscopy) were used to demonstrate that the dispersity in crystal size, shape and morphology increases with increasing calcium and oxalate ion input concentrations into the system. Additionally, CaOx crystals formed in high oxalate ion environments were found to be small in size when compared to those created in a calcium-rich environment. CaOx crystallization experiments involving a variety of chemical species show that this system can readily be applied to establish the influence of specific entities on a crystallization process. Design improvements, based on the obtained results, are outlined.