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The Fischer 344 spinal cord contusion model and effects of cyclosporin-A on the model
Descriptive
TitleInfo
Title
The Fischer 344 spinal cord contusion model and effects of cyclosporin-A on the model
Name
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Hsu
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Shao-Yun
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1983-
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Shao-Yun Hsu
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author
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Young
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Wise Young
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chair
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Grumet
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Martin
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Martin Grumet
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Advisory Committee
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co-chair
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Hsu
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Shu-Chan
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Shu-Chan Hsu
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Advisory Committee
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co-chair
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Bresnahan
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Jacqueline
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Jacqueline Bresnahan
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Advisory Committee
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outside member
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Rutgers University
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degree grantor
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Graduate School - New Brunswick
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school
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theses
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2016
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2016-05
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2016
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xx
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eng
Abstract
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The Multicenter Animal Spinal Cord Injury Study (MASCIS) standardized spinal cord injury (SCI) in Long-Evans hooded and Sprague-Dawley rats. In this thesis, we extended the MASCIS model to Fischer 344 (F344) rats for the following reasons. First, F344 rats are immune-compatible with each other. Cells can be transplanted from one F344 rat to another without immunosuppression. Second, our laboratory developed a strain of F344 rats that express green fluorescent protein (GFP). When transplanted into regular F344 rats, GFP F344 rat cells can be readily identified. Third, F344 rats are isogenic. They should have more uniform and replicable responses to injury and therapies. Finally, this F344 model allows us to assess effects of allogeneic cell transplants with and without immunosuppressive drugs, such as cyclosporin-A (CsA), as well as the effects of immunosuppressive drugs on transplanted cells. We chose to assess olfactory ensheathing glial (OEG) cell transplants because many investigators have reported that these cells improve motor recovery in rat SCI models. Our results show that F344 rats can be reproducibly injured with the MASCIS model and that OEG transplants significantly improve locomotor recovery when transplanted without CsA. However, we encountered several unexpected findings. Female rats recovered more than male rats even though the animals were age-matched, studies of the spinal cords revealed no difference in size and anatomy of age-matched male and female rats, and 24-hour lesion volumes in male and female spinal cords were the same. OEG transplants improved recovery of male rats, almost as well as untreated female rats. CsA therapy improved recovery in both male and female rats. We conclude that the MASCIS model causes reproducible injury to spinal cords of F344 rats, age-matched female rats recover better locomotor function than male rats, OEG cells can be transplanted from GFP F344 rats to wild-type F344 rats without immune rejection, OEG transplants improve functional recovery particularly in male rats, CsA improved locomotor recovery in both male and female F344 rats. The MASCIS impactor model should be very useful for assessing mechanisms underlying gender differences, OEG and CsA effects on transplanted cells and recovery mechanisms.
Subject
(authority = RUETD)
Topic
Neuroscience
Subject
(authority = ETD-LCSH)
Topic
Cyclosporine
Subject
(authority = ETD-LCSH)
Topic
Spinal cord--Wounds and injuries
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Rutgers University Electronic Theses and Dissertations
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ETD_7164
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electronic resource
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1 online resource (xvi, 154 p. : ill.)
Note
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Ph.D.
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Includes bibliographical references
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by Shao-Yun Hsu
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Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
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doi:10.7282/T35X2C3M
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ETD doctoral
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Rights
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The author owns the copyright to this work.
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Name
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Hsu
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Shao-Yun
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Permission or license
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2016-04-11 14:40:46
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Shao-Yun Hsu
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Rutgers University. Graduate School - New Brunswick
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Author Agreement License
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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2016-05-31
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2017-05-31
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Access to this PDF has been restricted at the author's request. It will be publicly available after May 31st, 2017.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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2016-04-11T18:05:43
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