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Probing the nature of AFEX-pretreated corn stover derived decomposition products that inhibit cellulase activity

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TypeOfResource
Text
TitleInfo
Title
Probing the nature of AFEX-pretreated corn stover derived decomposition products that inhibit cellulase activity
Name (type = corporate); (authority = RutgersOrg-Department)
NamePart
Chemical & Biochemical Engineering
Name (type = corporate); (authority = RutgersOrg-School)
NamePart
School of Engineering
Genre (authority = RULIB-FS)
Article, Non-refereed
Genre (authority = NISO JAV)
Author's original (AO)
OriginInfo
DateIssued (encoding = w3cdtf); (keyDate = yes)
2014
PhysicalDescription
InternetMediaType
application/pdf
Extent
44 p.
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
Subject (authority = local)
Topic
AFEX
Subject (authority = local)
Topic
Ultra-filtration
Subject (authority = local)
Topic
Fractionation
Subject (authority = local)
Topic
Cellulase
Subject (authority = local)
Topic
Inhibition
Abstract (type = abstract)
Sequential fractionation of AFEX-pretreated corn stover extracts was carried out using ultra-centrifugation, ultra-filtration, and solid phase extraction to isolate various classes of pretreatment products to evaluate their inhibitory effect on cellulases. Ultra-centrifugation removed dark brown precipitates that caused no appreciable enzyme inhibition. Ultra-filtration of ultra-centrifuged AFEX-pretreated corn stover extractives using a 10 kDa molecular weight cutoff (MWCO) membrane removed additional high molecular weight components that accounted for 24–28% of the total observed enzyme inhibition while a 3 kDa MWCO membrane removed 60–65%, suggesting significant inhibition is caused by oligomeric materials. Solid phase extraction (SPE) of AFEX-pretreated corn stover extractives after ultra-centrifugation removed 34–43% of the inhibition; ultra-filtration with a 5 kDa membrane removed 44–56% of the inhibition and when this ultra-filtrate was subjected to SPE a total of 69–70% of the inhibition were removed. Mass spectrometry found several phenolic compounds among the hydrophobic inhibition removed by SPE adsorption.
Name (type = personal)
NamePart (type = family)
Humpula
NamePart (type = given)
James F.
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Uppugundla
NamePart (type = given)
Nirmal
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Vismeh
NamePart (type = given)
Ramin
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Sousa
NamePart (type = given)
Leonardo
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal); (authority = orcid); (authorityURI = http://id.loc.gov/vocabulary/identifiers/orcid.html); (valueURI = http://orcid.org/0000-0003-3677-6735)
NamePart (type = family)
Chundawat
NamePart (type = given)
Shishir P. S.
Affiliation
Michigan State University; University of Wisconsin–Madison
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Jones
NamePart (type = given)
A. Daniel
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Balan
NamePart (type = given)
Venkatesh
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Dale
NamePart (type = given)
Bruce E.
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Cheh
NamePart (type = given)
Albert M.
Affiliation
American University
Role
RoleTerm (type = text); (authority = marcrt)
author
Extension
DescriptiveEvent
Type
Citation
DateTime (encoding = w3cdtf)
2014
AssociatedObject
Type
Journal
Relationship
Has part
Name
Bioresource Technology
Identifier (type = volume and issue)
152
Reference (type = url)
http://dx.doi.org/10.1016/j.biortech.2013.10.082
Detail
38-45
Note (type = special display note)
Published version: Humpula, James F., Uppugundla, Nirmal, Vismeh, Ramin, Sousa, Leonardo, Chundawat, Shishir P. S., Jones, A. Daniel, Balan, Venkatesh, Dale, Bruce E. & Cheh, Albert M. (2014). Probing the nature of AFEX-retreated corn stover derived decomposition products that inhibit cellulase activity. Bioresource Technology 152, 38-45. http://dx.doi.org/10.1016/j.biortech.2013.10.082
RelatedItem (type = host)
TitleInfo
Title
Chundawat, Shishir P. S.
Identifier (type = local)
rucore30218100001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3T43WCR
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Name
Multiple author license v. 1
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