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Profiling of Soluble Neutral Oligosaccharides from Treated Biomass using Solid Phase Extraction and Liquid Chromatography-Multiplexed Collision Induced Dissociation-Mass Spectrometry

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Text
TitleInfo
Title
Profiling of Soluble Neutral Oligosaccharides from Treated Biomass using Solid Phase Extraction and Liquid Chromatography-Multiplexed Collision Induced Dissociation-Mass Spectrometry
TitleInfo (type = alternative)
Title
Profiling of Soluble Neutral Oligosaccharides from Treated Biomass using Solid Phase Extraction and LC-TOF MS
Name (type = corporate); (authority = RutgersOrg-Department)
NamePart
Chemical & Biochemical Engineering
Name (type = corporate); (authority = RutgersOrg-School)
NamePart
School of Engineering
Genre (authority = RULIB-FS)
Article, Non-refereed
Genre (authority = NISO JAV)
Author's original (AO)
OriginInfo
DateIssued (encoding = w3cdtf); (keyDate = yes)
2013
PhysicalDescription
InternetMediaType
application/pdf
Extent
30 p.
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
Subject (authority = local)
Topic
Ammonia fiber expansion (AFEX) pretreatment
Subject (authority = local)
Topic
Arabinoxylans
Subject (authority = local)
Topic
Corn stover
Subject (authority = local)
Topic
Glucans
Subject (authority = local)
Topic
Porous graphitized carbon (PGC)
Subject (authority = local)
Topic
Solid phase extraction (SPE)
Abstract (type = abstract)
Thermochemical pretreatment of cellulosic biomass improves cell wall enzymatic digestibility, while simultaneously releasing substantial amounts of soluble oligosaccharides. Profiling of oligosaccharides released during pretreatment yield information essential for choosing glycosyl hydrolases necessary for cost-effective conversion of cellulosic biomass to desired biofuel/biochemical end-products. In this report we present a methodology for profiling of soluble neutral oligosaccharides released from ammonia fiber expansion (AFEX<sup>TM</sup>)-pretreated corn stover. Our methodology employs solid phase extraction (SPE) enrichment of oligosaccharides based on porous graphitized carbon (PGC), followed by high performance liquid chromatography (HPLC) separation using a polymeric amine based column (Prevail Carbohydrate ES) and electrospray ionization time-of-flight mass spectrometry (ESI-TOF-MS) in both positive and negative modes. For structural elucidation on the chromatographic time scale, nonselective multiplexed collision-induced dissociation was performed for quasi-simultaneous acquisition of accurate molecular and fragment masses of neutral oligosaccharids in a single analysis. These analyses directly revealed presence of glucans up to degree of polymerization (DP) 22 without side-chain modifications. Additionally, arabinoxylans with DP up to 6 were detected in the pretreated biomass samples (post-enzymatic digestion). All linkages between sugar units in glucans and arabinoxylans were identified to be p-1-4 linkages based on cross-ring fragment masses. Comprehensive profiling of soluble oligosaccharides also demonstrated that arabinoxylan acetylation was reduced by greater than 85% post-AFEX<sup>TM</sup> treatment.
Name (type = personal)
NamePart (type = family)
Vismeh
NamePart (type = given)
Ramin
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Humpula
NamePart (type = given)
James F.
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal); (authority = orcid); (authorityURI = http://id.loc.gov/vocabulary/identifiers/orcid.html); (valueURI = http://orcid.org/0000-0003-3677-6735)
NamePart (type = family)
Chundawat
NamePart (type = given)
Shishir P. S.
Affiliation
Michigan State University; University of Wisconsin--Madison
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Balan
NamePart (type = given)
Venkatesh
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Dale
NamePart (type = given)
Bruce E.
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Jones
NamePart (type = given)
A. Daniel
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Extension
DescriptiveEvent
Type
Citation
DateTime (encoding = w3cdtf)
2013
AssociatedObject
Type
Journal
Relationship
Has part
Name
Carbohydrate Polymers
Identifier (type = volume and issue)
94(2)
Reference (type = url)
http://dx.doi.org/10.1016/j.carbpol.2013.02.005
Detail
791-799
Note (type = special display note)
Published version: Vismeh, Ramin, Humpula, James F., Chundawat, Shishir P. S., Balan, Venkatesh, Dale, Bruce E. & Jones, A. Daniel. (2013). Profiling of Soluble Neutral Oligosaccharides from Treated Biomass using Solid Phase Extraction and LC-TOF MS. Carbohydrate Polymers 94(2), 791-799. http://dx.doi.org/10.1016/j.carbpol.2013.02.005
RelatedItem (type = host)
TitleInfo
Title
Chundawat, Shishir P. S.
Identifier (type = local)
rucore30218100001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3JM2CXP
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Name
Multiple author license v. 1
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2016-11-04T16:31:30
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
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