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Strategy for identification of novel fungal and bacterial glycosyl hydrolase hybrid mixtures that can efficiently saccharify pretreated lignocellulosic biomass

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TypeOfResource
Text
TitleInfo
Title
Strategy for identification of novel fungal and bacterial glycosyl hydrolase hybrid mixtures that can efficiently saccharify pretreated lignocellulosic biomass
Name (type = corporate); (authority = RutgersOrg-Department)
NamePart
Chemical & Biochemical Engineering
Name (type = corporate); (authority = RutgersOrg-School)
NamePart
School of Engineering
Genre (authority = RULIB-FS)
Article, Non-refereed
Genre (authority = NISO JAV)
Author's original (AO)
OriginInfo
DateIssued (encoding = w3cdtf); (keyDate = yes)
2010
PhysicalDescription
InternetMediaType
application/pdf
Extent
30 p.
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
Subject (authority = local)
Topic
AFEX
Subject (authority = local)
Topic
Enzymatic hydrolysis
Subject (authority = local)
Topic
Ethanol
Subject (authority = local)
Topic
Glycosyl hydrolases
Subject (authority = local)
Topic
Lignocellulose
Abstract (type = abstract)
We have applied a rational four-step strategy to identify novel bacterial glycosyl hydrolases (GH), in combination with various fungal enzymes, in order to define an efficient enzyme cocktail to hydrolyze pretreated lignocellulosic biomass. The fungal cellulases include cellobiohydrolase I (CBH I; GH family 7A), cellobiohydrolase II (CBH II; GH family 6A), endoglucanase I (EG I; GH family 7B) and ß-glucosidase (ßG; GH family 3). Bacterial endocellulases (LC1 and LC2; GH family 5), ß-glucosidase (LßG; GH family 1), endoxylanases (LX1 and LX2; GH family 10) and ß-xylosidase (LßX; GH family 52) from multiple sources were cloned, expressed and purified as well. Enzymatic hydrolysis for various enzyme combinations was performed on Ammonia Fiber Expansion (AFEX) treated corn stover at various total protein loadings (30, 15 and 10 mg enzyme/g glucan). The optimal mass ratio of enzymes necessary for obtaining high glucan and xylan yields was determined using a suitable mixture design of experiments. The optimal hybrid enzyme mixtures contain fungal cellulases (78% of total protein loading), which include CBH I (loading ranging between 9-51% of total enzyme load), CBH II (9-51%), EG I (10-50%), and bacterial hemicellulases (22% of total protein loading) comprising of LXl (13%) and LßX (9%). The hybrid mixture works effectively at 50 °C and pH 4.5 to efficiently saccharify AFEX treated corn stover giving as high as 95% glucan and 65% xylan conversion, respectively. This strategy of screening enzyme mixtures on pretreated lignocellulose will ultimately help develop enzyme cocktails that can hydrolyze plant cell walls efficiently and economically to produce cellulosic ethanol.
Name (type = personal)
NamePart (type = family)
Gao
NamePart (type = given)
Dahai
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal); (authority = orcid); (authorityURI = http://id.loc.gov/vocabulary/identifiers/orcid.html); (valueURI = http://orcid.org/0000-0003-3677-6735)
NamePart (type = family)
Chundawat
NamePart (type = given)
Shishir P. S.
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Liu
NamePart (type = given)
Tongjun
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Hermanson
NamePart (type = given)
Spencer
Affiliation
Lucigen Corporation; Great Lakes Bioenergy Research Center (GLBRC), Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Gowda
NamePart (type = given)
Krishne
Role
RoleTerm (type = text); (authority = marcrt)
author
Affiliation
Lucigen Corporation; Great Lakes Bioenergy Research Center (GLBRC), Michigan State University
Name (type = personal)
NamePart (type = family)
Brumm
NamePart (type = given)
Phillip
Role
RoleTerm (type = text); (authority = marcrt)
author
Affiliation
Lucigen Corporation; Great Lakes Bioenergy Research Center (GLBRC), Michigan State University
Name (type = personal)
NamePart (type = family)
Dale
NamePart (type = given)
Bruce E.
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Balan
NamePart (type = given)
Venkatesh
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Extension
DescriptiveEvent
Type
Citation
DateTime (encoding = w3cdtf)
2010
AssociatedObject
Type
Journal
Relationship
Has part
Name
BioEnergy Research
Identifier (type = volume and issue)
3(1)
Reference (type = url)
http://dx.doi.org/10.1007/s12155-009-9066-6
Detail
67–81
Note (type = special display note)
The final publication (Version of Record) is available at Springer via http://dx.doi.org/10.1007/s12155-009-9066-6
RelatedItem (type = host)
TitleInfo
Title
Chundawat, Shishir P. S.
Identifier (type = local)
rucore30218100001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T35B04RM
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Multiple author license v. 1
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2016-11-04T16:38:14
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
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