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Hemicellulases and auxiliary enzymes for improved conversion of lignocellulosic biomass to monosaccharides

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TypeOfResource
Text
TitleInfo
Title
Hemicellulases and auxiliary enzymes for improved conversion of lignocellulosic biomass to monosaccharides
Name (authority = RutgersOrg-Department); (type = corporate)
NamePart
Chemical & Biochemical Engineering
Genre (authority = RULIB-FS)
Article, Refereed
Genre (authority = NISO JAV)
Accepted Manuscript (AM)
OriginInfo
DateIssued (encoding = w3cdtf); (keyDate = yes)
2011
PhysicalDescription
InternetMediaType
application/pdf
Extent
34 p.
Extension
DescriptiveEvent
Type
Citation
DateTime (encoding = w3cdtf)
2011
AssociatedObject
Type
Journal
Relationship
Has part
Name
Biotechnology for Biofuels
Identifier (type = volume and issue)
4(5)
Reference (type = url)
http://dx.doi.org/10.1186/1754-6834-4-5
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
Subject (authority = local)
Topic
Enzymatic-hydrolysis
Subject (authority = local)
Topic
Corn stover
Subject (authority = local)
Topic
Technologies
Subject (authority = local)
Topic
Pretreatment
Subject (authority = local)
Topic
AFEX
Subject (authority = local)
Topic
Cellulose
Subject (authority = local)
Topic
Ethanol
Subject (authority = local)
Topic
Systems
Abstract (type = abstract)
Background
High enzyme loading is a major economic bottleneck for the commercial processing of pretreated lignocellulosic biomass to produce fermentable sugars. Optimizing the enzyme cocktail for specific types of pretreated biomass allows for a significant reduction in enzyme loading without sacrificing hydrolysis yield. This is especially important for alkaline pretreatments such as Ammonia fiber expansion (AFEX) pretreated corn stover. Hence, a diverse set of hemicellulases supplemented along with cellulases is necessary for high recovery of monosaccharides.

Results
The core fungal cellulases in the optimal cocktail include cellobiohydrolase I [CBH I; glycoside hydrolase (GH) family 7A], cellobiohydrolase II (CBH II; GH family 6A), endoglucanase I (EG I; GH family 7B) and β-glucosidase (βG; GH family 3). Hemicellulases tested along with the core cellulases include xylanases (LX1, GH family 10; LX2, GH family 10; LX3, GH family 10; LX4, GH family 11; LX5, GH family 10; LX6, GH family 10), β-xylosidase (LβX; GH family 52), α-arabinofuranosidase (LArb, GH family 51) and α-glucuronidase (LαGl, GH family 67) that were cloned, expressed and/or purified from different bacterial sources. Different combinations of these enzymes were tested using a high-throughput microplate based 24 h hydrolysis assay. Both family 10 (LX3) and family 11 (LX4) xylanases were found to most efficiently hydrolyze AFEX pretreated corn stover in a synergistic manner. The optimal mass ratio of xylanases (LX3 and LX4) to cellulases (CBH I, CBH II and EG I) is 25:75. LβX (0.6 mg/g glucan) is crucial to obtaining monomeric xylose (54% xylose yield), while LArb (0.6 mg/g glucan) and LαGl (0.8 mg/g glucan) can both further increase xylose yield by an additional 20%. Compared with Accellerase 1000, a purified cocktail of cellulases supplemented with accessory hemicellulases will not only increase both glucose and xylose yields but will also decrease the total enzyme loading needed for equivalent yields.

Conclusions
A diverse set of accessory hemicellulases was found necessary to enhance the synergistic action of cellulases hydrolysing AFEX pretreated corn stover. High glucose (around 80%) and xylose (around 70%) yields were achieved with a moderate enzyme loading (~20 mg protein/g glucan) using an in-house developed cocktail compared to commercial enzymes.
Note (type = peerReview)
Peer reviewed.
Name (type = personal)
NamePart (type = family)
Gao
NamePart (type = given)
Dahai
Affiliation
Michigan State University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Uppugundla
NamePart (type = given)
Nirmal
Affiliation
Michigan State University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (authority = orcid); (authorityURI = http://id.loc.gov/vocabulary/identifiers/orcid.html); (type = personal); (valueURI = http://orcid.org/0000-0003-3677-6735)
NamePart (type = family)
Chundawat
NamePart (type = given)
Shishir P. S.
Affiliation
Michigan State University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Yu
NamePart (type = given)
Xiurong
Affiliation
JiLin Rorgoo Renewable Energy Development Co Ltd; Biomass Conversion Research Lab (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Hermanson
NamePart (type = given)
Spencer
Affiliation
Lucigen Corporation; Great Lakes Bioenergy Research Center (GLBRC), Michigan State University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Gowda
NamePart (type = given)
Krishne
Role
RoleTerm (authority = marcrt); (type = text)
author
Affiliation
Lucigen Corporation; Great Lakes Bioenergy Research Center (GLBRC), Michigan State University
Name (type = personal)
NamePart (type = family)
Brumm
NamePart (type = given)
Phillip
Role
RoleTerm (authority = marcrt); (type = text)
author
Affiliation
Lucigen Corporation; Great Lakes Bioenergy Research Center (GLBRC), Michigan State University
Name (type = personal)
NamePart (type = family)
Mead
NamePart (type = given)
David
Role
RoleTerm (authority = marcrt); (type = text)
author
Affiliation
Lucigen Corporation; Great Lakes Bioenergy Research Center (GLBRC), Michigan State University
Name (type = personal)
NamePart (type = family)
Balan
NamePart (type = given)
Venkatesh
Affiliation
Michigan State University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Dale
NamePart (type = given)
Bruce E.
Affiliation
Michigan State University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (authority = RutgersOrg-School); (type = corporate)
NamePart
School of Engineering
RelatedItem (type = host)
TitleInfo
Title
Chundawat, Shishir P. S.
Identifier (type = local)
rucore30218100001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T31J9D26
Genre (authority = ExL-Esploro)
Accepted Manuscript
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Multiple author license v. 1
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ContentModel
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1.3
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Mac OS X 10.11.6 Quartz PDFContext
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2016-11-04T16:41:10
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