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Optimization of ammonia fiber expansion (AFEX) pretreatment and enzymatic hydrolysis of Miscanthus x giganteus to fermentable sugars

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TypeOfResource
Text
TitleInfo
Title
Optimization of ammonia fiber expansion (AFEX) pretreatment and enzymatic hydrolysis of Miscanthus x giganteus to fermentable sugars
Genre (authority = RULIB-FS)
Article, Non-refereed
Genre (authority = NISO JAV)
Author's original (AO)
OriginInfo
DateIssued (encoding = w3cdtf); (keyDate = yes)
2007
PhysicalDescription
InternetMediaType
application/pdf
Extent
27 p.
Extension
DescriptiveEvent
Type
Citation
DateTime (encoding = w3cdtf)
2007
AssociatedObject
Type
Journal
Relationship
Has part
Name
Biotechnology Progress
Identifier (type = volume and issue)
23(4)
Reference (type = url)
http://dx.doi.org/10.1021/bp070098m
Detail
846-850
Name (type = personal)
NamePart (type = family)
Murnen
NamePart (type = given)
Hannah K.
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Balan
NamePart (type = given)
Venkatesh
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal); (authority = orcid); (authorityURI = http://id.loc.gov/vocabulary/identifiers/orcid.html); (valueURI = http://orcid.org/0000-0003-3677-6735)
NamePart (type = family)
Chundawat
NamePart (type = given)
Shishir P. S.
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Bals
NamePart (type = given)
Bryan
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Sousa
NamePart (type = given)
Leonardo
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Dale
NamePart (type = given)
Bruce E.
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
Subject (authority = local)
Topic
Miscanthus
Subject (authority = local)
Topic
AFEX pretreatment
Subject (authority = local)
Topic
Enzymatic hydrolysis
Subject (authority = local)
Topic
Biomass
Subject (authority = local)
Topic
Bio-refinery
Abstract (type = abstract)
Miscanthus x giganteus is a tall perennial grass whose suitability as an energy crop is presently being appraised. There is very little information on the effect of pretreatment and enzymatic saccharification of Miscanthus to produce fermentable sugars. This paper reports sugar yields during enzymatic hydrolysis from ammonia fiber expansion (AFEX) pretreated Miscanthus. Pretreatment conditions including temperature, moisture, ammonia loading, residence time, and enzyme loadings are varied to maximize hydrolysis yields. In addition, further treatments such as soaking the biomass prior to AFEX as well as washing the pretreated material were also attempted to improve sugar yields. The optimal AFEX conditions determined were 160 °C, 2:1 (w/w) ammonia to biomass loading, 233% moisture (dry weight basis), and 5 min reaction time for water-soaked Miscanthus. Approximately 96% glucan and 81% xylan conversions were achieved after 168 h enzymatic hydrolysis at 1% glucan loading using 15 FPU/(g of glucan) of cellulase and 64 p-NPGU/(g of glucan) of β-glucosidase along with xylanase and tween-80 supplementation. A mass balance for the AFEX pretreatment and enzymatic hydrolysis process is presented.
Note (type = special display note)
The final publication (Version of Record) is available at http://dx.doi.org/10.1021/bp070098m
Name (type = corporate); (authority = RutgersOrg-School)
NamePart
School of Engineering
Name (type = corporate); (authority = RutgersOrg-Department)
NamePart
Chemical & Biochemical Engineering
RelatedItem (type = host)
TitleInfo
Title
Chundawat, Shishir P. S.
Identifier (type = local)
rucore30218100001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T34X5BPN
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Copyright for scholarly resources published in RUcore is retained by the copyright holder. By virtue of its appearance in this open access medium, you are free to use this resource, with proper attribution, in educational and other non-commercial settings. Other uses, such as reproduction or republication, may require the permission of the copyright holder.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsEvent
Type
Permission or license
AssociatedObject
Type
License
Name
Multiple author license v. 1
Detail
I hereby grant to Rutgers, The State University of New Jersey (Rutgers) the non-exclusive right to retain, reproduce, and distribute the deposited work (Work) in whole or in part, in and from its electronic format, without fee. This agreement does not represent a transfer of copyright to Rutgers.Rutgers may make and keep more than one copy of the Work for purposes of security, backup, preservation, and access and may migrate the Work to any medium or format for the purpose of preservation and access in the future. Rutgers will not make any alteration, other than as allowed by this agreement, to the Work.I represent and warrant to Rutgers that the Work is my original work. I also represent that the Work does not, to the best of my knowledge, infringe or violate any rights of others.I further represent and warrant that I have obtained all necessary rights to permit Rutgers to reproduce and distribute the Work and that any third-party owned content is clearly identified and acknowledged within the Work.By granting this license, I acknowledge that I have read and agreed to the terms of this agreement and all related RUcore and Rutgers policies.
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Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
Document
CreatingApplication
Version
1.4
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Mac OS X 10.11.6 Quartz PDFContext
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2016-11-04T16:57:48
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2016-11-04T16:57:48
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