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A comparative study of ethanol production using dilute acid, ionic liquid and AFEX™ pretreated corn stover

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Text
TitleInfo
Title
A comparative study of ethanol production using dilute acid, ionic liquid and AFEX™ pretreated corn stover
Name (type = corporate); (authority = RutgersOrg-Department)
NamePart
Chemical & Biochemical Engineering
Name (type = corporate); (authority = RutgersOrg-School)
NamePart
School of Engineering
Genre (authority = RULIB-FS)
Article, Refereed
Genre (authority = NISO JAV)
Version of Record (VoR)
OriginInfo
DateIssued (encoding = w3cdtf); (keyDate = yes)
2014
PhysicalDescription
InternetMediaType
application/pdf
Extent
14 p.
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
Subject (authority = local)
Topic
AFEX
Subject (authority = local)
Topic
Dilute acid
Subject (authority = local)
Topic
Ionic liquid
Subject (authority = local)
Topic
Pretreatment
Subject (authority = local)
Topic
Enzymatic hydrolysis
Subject (authority = local)
Topic
Cellulosic ethanol
Abstract (type = abstract)
Background: In a biorefinery producing cellulosic biofuels, biomass pretreatment will significantly influence the efficacy of enzymatic hydrolysis and microbial fermentation. Comparison of different biomass pretreatment technigues by studying the impact of pretreatment on downstream operations at industrially relevant conditions and performing comprehensive mass balances will help focus attention on necessary process improvements, and thereby help reduce the cost of biofuel production.

Results: An on-going collaboration between the three US Department of Energy (DOE) funded bioenergy research centers (Great Lakes Bioenergy Research Center (GLBRC), Joint BioEnergy Institute (JBEI) and BioEnergy Science Center (BESQ) has given us a unigue opportunity to compare the performance of three pretreatment processes, notably dilute acid (DA), ionic liguid (IL) and ammonia fiber expansion (AFEX™), using the same source of com stover. Separate hydrolysis and fermentation (SHF) was carried out using various combinations of commercially available enzymes and engineered yeast (Saccharomyces cerevlslae 424A) strain. The optimal commercial enzyme combination (Ctec2: Htec2: Multifect Pectinase, percentage total protein loading basis) was evaluated for each pretreatment with a microplate-based assay using milled pretreated solids at 0.2% glucan loading and 15 mg total protein loading/g of glucan. The best enzyme combinations were 67:33:0 for DA, 39:33:28 for IL and 67:17:17 for AFEX. The amounts of sugar (kg) (glucose: xylose: total gluco- and xylo-oligomers) per 100 kg of untreated com stover produced after 72 hours of 6% glucan loading enzymatic hydrolysis were: DA (25:2:2), IL (31:15:2) and AFEX (26:13:7). Additionally, the amounts of ethanol (kg) produced per 100 kg of untreated com stover and the respective ethanol metabolic yield (%) achieved with exogenous nutrient supplemented fermentations were: DA (14.0, 92.0%), IL (21.2, 93.0%) and AFEX (20.5, 95.0%), respectively. The reason for lower ethanol yield for DA is because most of the xylose produced during the pretreatment was removed and not converted to ethanol during fermentation.

Conclusions: Compositional analysis of the pretreated biomass solids showed no significant change in composition for AFEX treated com stover, while about 85% of hemicellulose was solubilized after DA pretreatment, and about 90% of lignin was removed after IL pretreatment. As expected, the optimal commercial enzyme combination was different for the solids prepared by different pretreatment technologies. Due to loss of nutrients during the pretreatment and washing steps, DA and IL pretreated hydrolysates reguired exogenous nutrient supplementation to ferment glucose and xylose efficiently, while AFEX pretreated hydrolysate did not reguire nutrient supplementation.
Note (type = peerReview)
Peer reviewed.
Name (type = personal)
NamePart (type = family)
Uppugundla
NamePart (type = given)
Nirmal
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Sousa
NamePart (type = given)
Leonardo
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal); (authority = orcid); (authorityURI = http://id.loc.gov/vocabulary/identifiers/orcid.html); (valueURI = http://orcid.org/0000-0003-3677-6735)
NamePart (type = family)
Chundawat
NamePart (type = given)
Shishir P. S.
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Yu
NamePart (type = given)
Xiurong
Affiliation
Jilin TuoPai Agriculture Products Development Ltd
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Simmons
NamePart (type = given)
Blake A.
Affiliation
Joint BioEnergy Institute (JBEI) and Sandia National Laboratories
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Singh
NamePart (type = given)
Seema
Role
RoleTerm (type = text); (authority = marcrt)
author
Affiliation
Joint BioEnergy Institute (JBEI) and Sandia National Laboratories
Name (type = personal)
NamePart (type = family)
Gao
NamePart (type = given)
Xiadi
Affiliation
Oak Ridge National Laboratory; University of California, Riverside
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Kumar
NamePart (type = given)
Rajeev
Role
RoleTerm (type = text); (authority = marcrt)
author
Affiliation
Oak Ridge National Laboratory; University of California, Riverside
Name (type = personal)
NamePart (type = family)
Wyman
NamePart (type = given)
Charles E.
Role
RoleTerm (type = text); (authority = marcrt)
author
Affiliation
Oak Ridge National Laboratory; University of California, Riverside
Name (type = personal)
NamePart (type = family)
Dale
NamePart (type = given)
Bruce E.
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Balan
NamePart (type = given)
Venkatesh
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Extension
DescriptiveEvent
Type
Citation
DateTime (encoding = w3cdtf)
2014
AssociatedObject
Type
Journal
Relationship
Has part
Name
Biotechnology for Biofuels
Identifier (type = volume and issue)
7(72)
Reference (type = url)
http://dx.doi.org/10.1186/1754-6834-7-72
RelatedItem (type = host)
TitleInfo
Title
Chundawat, Shishir P. S.
Identifier (type = local)
rucore30218100001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3BV7JV9
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