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The quest for alternatives to microbial cellulase mix production: corn stover-produced heterologous multi-cellulases readily deconstruct lignocellulosic biomass into fermentable sugars

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TypeOfResource
Text
TitleInfo
Title
The quest for alternatives to microbial cellulase mix production: corn stover-produced heterologous multi-cellulases readily deconstruct lignocellulosic biomass into fermentable sugars
Name (type = corporate); (authority = RutgersOrg-Department)
NamePart
Chemical & Biochemical Engineering
Genre (authority = RULIB-FS)
Article, Refereed
Genre (authority = NISO JAV)
Accepted Manuscript (AM)
OriginInfo
DateIssued (encoding = w3cdtf); (keyDate = yes)
2011
PhysicalDescription
InternetMediaType
application/pdf
Extent
32 p.
Extension
DescriptiveEvent
Type
Citation
DateTime (encoding = w3cdtf)
2011
AssociatedObject
Type
Journal
Relationship
Has part
Name
Journal of Chemical Technology and Biotechnology
Identifier (type = volume and issue)
86(5)
Reference (type = url)
http://dx.doi.org/10.1002/jctb.2584
Detail
633–641
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
Subject (authority = local)
Topic
El
Subject (authority = local)
Topic
Endo-cellulase
Subject (authority = local)
Topic
CBH I
Subject (authority = local)
Topic
1,4-β-cellobiohydrolases I
Subject (authority = local)
Topic
Cellobiase
Subject (authority = local)
Topic
Corn
Subject (authority = local)
Topic
Maize
Subject (authority = local)
Topic
Fermentable sugar
Subject (authority = local)
Topic
Biofuels
Subject (authority = local)
Topic
AFEX
Abstract (type = abstract)
BACKGROUND: Production of cellulosic ethanol is still expensive compared with corn (maize) grain ethanol due to the high costs of bulk production of microbial cellulases. At least three cellulases including endo-cellulase, exo-cellulase and cellobiase are needed to convert cellulosic biomass into fermentable sugars. All these cellulases could be self-produced within cells of transgenic bio-energy crops. The production of heterologous Acidothermus cellulolyticus (E1) endo-cellulase in endoplasmic reticulum and mitochondria of green tissues of transgenic corn plants was recently reported, and it was confirmed that the heterologous E1 converts cellulose into fermentable sugars.

RESULTS: Biologically active A. cellulolyticus E1, Trichoderma reesei 1,4-β-cellobiohydrolases I (CBH I) exo-cellulase and bovine rumen Butyrivibrio fibrisolvens cellobiase were expressed in corn plant endoplasmic reticulum (ER), apoplast (cell wall areas) and vacuole respectively. Results show that the ratio 1 : 4 : 1 (E1 : CBH I : cellobiase) of crude heterologous cellulases is ideal for converting ammonia fiber explosion (AFEX) pretreated corn stover into fermentable sugars.

CONCLUSIONS: Corn plants that express all three biologically active heterologous cellulases within their cellulosic biomass to facilitate conversion of pretreated corn stover into fermentable sugars is a step forward in the quest for alternatives to the present microbial cellulase mix production for cellulosic biofuels.
Note (type = peerReview)
Peer reviewed.
Name (type = personal)
NamePart (type = family)
Park
NamePart (type = given)
Sang-Hyuck
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Ransom
NamePart (type = given)
Callista
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Mei
NamePart (type = given)
Chuansheng
Affiliation
The Institute for Advanced Learning and Research
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Sabzikar
NamePart (type = given)
Robab
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Qi
NamePart (type = given)
Chunfang
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal); (authority = orcid); (authorityURI = http://id.loc.gov/vocabulary/identifiers/orcid.html); (valueURI = http://orcid.org/0000-0003-3677-6735)
NamePart (type = family)
Chundawat
NamePart (type = given)
Shishir P. S.
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Dale
NamePart (type = given)
Bruce E.
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Sticklen
NamePart (type = given)
Mariam
Affiliation
Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = corporate); (authority = RutgersOrg-School)
NamePart
School of Engineering
RelatedItem (type = host)
TitleInfo
Title
Chundawat, Shishir P. S.
Identifier (type = local)
rucore30218100001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3Q52RWV
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License
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Multiple author license v. 1
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2016-11-04T17:14:21
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2016-11-04T13:15:40
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