Staff View
Designer synthetic media for studying microbial-catalyzed biofuel production

Descriptive

TypeOfResource
Text
TitleInfo
Title
Designer synthetic media for studying microbial-catalyzed biofuel production
Name (type = corporate); (authority = RutgersOrg-Department)
NamePart
Chemical & Biochemical Engineering
Name (type = corporate); (authority = RutgersOrg-School)
NamePart
School of Engineering
Genre (authority = RULIB-FS)
Article, Refereed
Genre (authority = NISO JAV)
Version of Record (VoR)
OriginInfo
DateIssued (encoding = w3cdtf); (keyDate = yes)
2015
PhysicalDescription
InternetMediaType
application/pdf
Extent
17 p.
Extension
DescriptiveEvent
Type
Citation
DateTime (encoding = w3cdtf)
2015
AssociatedObject
Type
Journal
Relationship
Has part
Name
Biotechnology for Biofuels
Identifier (type = volume and issue)
8(1)
Reference (type = url)
http://dx.doi.org/10.1186/s13068-014-0179-6
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
Subject (authority = local)
Topic
Synthetic hydrolysate
Subject (authority = local)
Topic
Lignocellulose
Subject (authority = local)
Topic
AFEX
Subject (authority = local)
Topic
Yeast fermentation inhibition
Subject (authority = local)
Topic
Amides inhibition
Subject (authority = local)
Topic
Carboxylic acids inhibition
Subject (authority = local)
Topic
Pretreatment decomposition products
Subject (authority = local)
Topic
Hydrolysate composition
Abstract (type = abstract)
Background
The fermentation inhibition of yeast or bacteria by lignocellulose-derived degradation products, during hexose/pentose co-fermentation, is a major bottleneck for cost-effective lignocellulosic biorefineries. To engineer microbial strains for improved performance, it is critical to understand the mechanisms of inhibition that affect fermentative organisms in the presence of major components of a lignocellulosic hydrolysate. The development of a synthetic lignocellulosic hydrolysate (SH) media with a composition similar to the actual biomass hydrolysate will be an important advancement to facilitate these studies. In this work, we characterized the nutrients and plant-derived decomposition products present in AFEX™ pretreated corn stover hydrolysate (ACH). The SH was formulated based on the ACH composition and was further used to evaluate the inhibitory effects of various families of decomposition products during Saccharomyces cerevisiae 424A (LNH-ST) fermentation.

Results
The ACH contained high levels of nitrogenous compounds, notably amides, pyrazines, and imidazoles. In contrast, a relatively low content of furans and aromatic and aliphatic acids were found in the ACH. Though most of the families of decomposition products were inhibitory to xylose fermentation, due to their abundance, the nitrogenous compounds showed the most inhibition. From these compounds, amides (products of the ammonolysis reaction) contributed the most to the reduction of the fermentation performance. However, this result is associated to a concentration effect, as the corresponding carboxylic acids (products of hydrolysis) promoted greater inhibition when present at the same molar concentration as the amides.

Due to its complexity, the formulated SH did not perfectly match the fermentation profile of the actual hydrolysate, especially the growth curve. However, the SH formulation was effective for studying the inhibitory effect of various compounds on yeast fermentation.

Conclusions
The formulation of SHs is an important advancement for future multi-omics studies and for better understanding the mechanisms of fermentation inhibition in lignocellulosic hydrolysates. The SH formulated in this work was instrumental for defining the most important inhibitors in the ACH. Major AFEX decomposition products are less inhibitory to yeast fermentation than the products of dilute acid or steam explosion pretreatments; thus, ACH is readily fermentable by yeast without any detoxification.
Note (type = peerReview)
Peer reviewed.
Name (type = personal)
NamePart (type = family)
Tang
NamePart (type = given)
Xiaoyu
Affiliation
Biogas Institute of Ministry of Agriculture
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Sousa
NamePart (type = given)
Leonardo
Affiliation
DOE Great Lakes Bioenergy Research Center, Biomass Conversion Research Lab (BCRL), Chemical Engineering and Materials Science, Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Jin
NamePart (type = given)
Mingjie
Affiliation
DOE Great Lakes Bioenergy Research Center, Biomass Conversion Research Lab (BCRL), Chemical Engineering and Materials Science, Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal); (authority = orcid); (authorityURI = http://id.loc.gov/vocabulary/identifiers/orcid.html); (valueURI = http://orcid.org/0000-0003-3677-6735)
NamePart (type = family)
Chundawat
NamePart (type = given)
Shishir P. S.
Affiliation
DOE Great Lakes Bioenergy Research Center, Biomass Conversion Research Lab (BCRL), Chemical Engineering and Materials Science, Michigan State University; Chemical & Biochemical Engineering, Rutgers University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Chambliss
NamePart (type = given)
Charles Kevin
Affiliation
Baylor University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Lau
NamePart (type = given)
Ming W.
Affiliation
DOE Great Lakes Bioenergy Research Center, Biomass Conversion Research Lab (BCRL), Chemical Engineering and Materials Science, Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Xiao
NamePart (type = given)
Zeyi
Affiliation
School of Chemical Engineering, Sichuan University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Dale
NamePart (type = given)
Bruce E.
Affiliation
DOE Great Lakes Bioenergy Research Center, Biomass Conversion Research Lab (BCRL), Chemical Engineering and Materials Science, Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Balan
NamePart (type = given)
Venkatesh
Affiliation
DOE Great Lakes Bioenergy Research Center, Biomass Conversion Research Lab (BCRL), Chemical Engineering and Materials Science, Michigan State University
Role
RoleTerm (type = text); (authority = marcrt)
author
RelatedItem (type = host)
TitleInfo
Title
Chundawat, Shishir P. S.
Identifier (type = local)
rucore30218100001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T39W0HRX
Back to the top

Rights

RightsDeclaration (AUTHORITY = FS); (ID = rulibRdec0004)
Copyright for scholarly resources published in RUcore is retained by the copyright holder. By virtue of its appearance in this open access medium, you are free to use this resource, with proper attribution, in educational and other non-commercial settings. Other uses, such as reproduction or republication, may require the permission of the copyright holder.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsEvent
Type
Permission or license
AssociatedObject
Type
License
Name
Multiple author license v. 1
Detail
I hereby grant to Rutgers, The State University of New Jersey (Rutgers) the non-exclusive right to retain, reproduce, and distribute the deposited work (Work) in whole or in part, in and from its electronic format, without fee. This agreement does not represent a transfer of copyright to Rutgers.Rutgers may make and keep more than one copy of the Work for purposes of security, backup, preservation, and access and may migrate the Work to any medium or format for the purpose of preservation and access in the future. Rutgers will not make any alteration, other than as allowed by this agreement, to the Work.I represent and warrant to Rutgers that the Work is my original work. I also represent that the Work does not, to the best of my knowledge, infringe or violate any rights of others.I further represent and warrant that I have obtained all necessary rights to permit Rutgers to reproduce and distribute the Work and that any third-party owned content is clearly identified and acknowledged within the Work.By granting this license, I acknowledge that I have read and agreed to the terms of this agreement and all related RUcore and Rutgers policies.
Back to the top

Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
Document
CreatingApplication
Version
1.3
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2016-11-04T17:19:18
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2016-11-04T13:21:24
ApplicationName
Mac OS X 10.11.6 Quartz PDFContext
Back to the top
Version 8.3.13
Rutgers University Libraries - Copyright ©2020