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Isolation of Lipid Droplets from Cells by Density Gradient Centrifugation

Descriptive

TypeOfResource
Text
TitleInfo
Title
Isolation of Lipid Droplets from Cells by Density Gradient Centrifugation
Identifier (type = PMID)
27580706
Name (type = personal); (authority = orcid); (authorityURI = http://id.loc.gov/vocabulary/identifiers/orcid.html); (valueURI = http://orcid.org/0000-0002-8553-8285)
NamePart (type = family)
Brasaemle
NamePart (type = given)
Dawn L.
Affiliation
Nutritional Sciences (School of Environmental and Biological Sciences, SEBS), Rutgers University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Wolins
NamePart (type = given)
Nathan E.
Affiliation
Washington University School of Medicine
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = corporate); (authority = RutgersOrg-Department)
NamePart
Nutritional Sciences (School of Environmental and Biological Sciences, SEBS)
Name (type = corporate); (authority = RutgersOrg-School)
NamePart
School of Environmental and Biological Sciences (SEBS)
Genre (authority = RULIB-FS)
Article, Refereed
Genre (authority = NISO JAV)
Accepted Manuscript (AM)
OriginInfo
DateIssued (encoding = w3cdtf); (keyDate = yes)
2016
Abstract (type = Abstract)
Lipid droplets are organelles found in most mammalian cells, as well as various plant tissues and yeast. They are composed of a core of neutral lipids surrounded by a membrane monolayer of phospholipids and cholesterol into which specific proteins are embedded. This unit provides protocols for isolating lipid droplets from mammalian cells by discontinuous density gradient centrifugation.
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
PhysicalDescription
InternetMediaType
application/pdf
Extent
17 p.
Subject (authority = local)
Topic
Cell cultures
Subject (authority = local)
Topic
Mammalian
Subject (authority = local)
Topic
Preparation of lipid droplets from centrifugation
Subject (authority = local)
Topic
Density-gradientcentrifugation
Subject (authority = local)
Topic
Isolation of lipid droplets
Extension
DescriptiveEvent
Type
Citation
DateTime (encoding = w3cdtf)
2016
AssociatedObject
Name
Current Protocols in Cell Biology
Type
Journal
Relationship
Has part
Detail
3.15.1-3.15.13
Reference (type = url)
http://dx.doi.org/10.1002/cpcb.10
Identifier (type = volume and issue)
72
Extension
DescriptiveEvent
Type
Grant award
AssociatedObject
Type
Grant number
Name
DK054797
AssociatedEntity
Role
Funder
Name
National Institutes of Health (U.S.)
AssociatedEntity
Role
Originator
Name
Dawn L. Brasaemle
Extension
DescriptiveEvent
Type
Grant award
AssociatedEntity
Role
Funder
Name
National Institutes of Health (U.S.)
AssociatedEntity
Role
Originator
Name
Nathan E. Wolins
AssociatedObject
Type
Grant number
Name
DK088206
RelatedItem (type = host)
TitleInfo
Title
Brasaemle, Dawn L.
Identifier (type = local)
rucore30219900001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3KK9F3C
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Rights

RightsDeclaration (AUTHORITY = FS); (ID = rulibRdec0004)
Copyright for scholarly resources published in RUcore is retained by the copyright holder. By virtue of its appearance in this open access medium, you are free to use this resource, with proper attribution, in educational and other non-commercial settings. Other uses, such as reproduction or republication, may require the permission of the copyright holder.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsEvent
Type
Permission or license
AssociatedObject
Type
License
Name
Multiple author license v. 1
Detail
I hereby grant to Rutgers, The State University of New Jersey (Rutgers) the non-exclusive right to retain, reproduce, and distribute the deposited work (Work) in whole or in part, in and from its electronic format, without fee. This agreement does not represent a transfer of copyright to Rutgers.Rutgers may make and keep more than one copy of the Work for purposes of security, backup, preservation, and access and may migrate the Work to any medium or format for the purpose of preservation and access in the future. Rutgers will not make any alteration, other than as allowed by this agreement, to the Work.I represent and warrant to Rutgers that the Work is my original work. I also represent that the Work does not, to the best of my knowledge, infringe or violate any rights of others.I further represent and warrant that I have obtained all necessary rights to permit Rutgers to reproduce and distribute the Work and that any third-party owned content is clearly identified and acknowledged within the Work.By granting this license, I acknowledge that I have read and agreed to the terms of this agreement and all related RUcore and Rutgers policies.
RightsEvent
Type
Embargo
DateTime (encoding = w3cdtf); (point = end); (qualifier = exact); (keyDate = no)
2017-09-01
DateTime (encoding = w3cdtf); (point = start); (qualifier = exact); (keyDate = no)
2016-09-01
Detail
Access to this PDF has been restricted at the publisher's request. It will be publicly available after: September 1, 2017.
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Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
Book
CreatingApplication
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1.4
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2016-10-14T02:43:30
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2016-10-14T12:13:39
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Antenna House PDF Output Library 6.2.553 (Windows (x64))
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