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Reductive dechlorination of polychlorinated dibenzo-p-dioxins by dehalococcoides-enriched cultures from contaminated soils and sediments
Descriptive
TitleInfo
Title
Reductive dechlorination of polychlorinated dibenzo-p-dioxins by dehalococcoides-enriched cultures from contaminated soils and sediments
Name
(type = personal)
NamePart
(type = family)
Dam
NamePart
(type = given)
Hang Thuy
NamePart
(type = date)
1983-
DisplayForm
Hang Thuy Dam
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Haggblom
NamePart
(type = given)
Max M
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Max M Haggblom
Affiliation
Advisory Committee
Role
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(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Kerkhof
NamePart
(type = given)
Lee J
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Lee J Kerkhof
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Fennell
NamePart
(type = given)
Donna E
DisplayForm
Donna E Fennell
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Rodenburg
NamePart
(type = given)
Lisa A
DisplayForm
Lisa A Rodenburg
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2016
DateOther
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2016-10
CopyrightDate
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2016
Place
PlaceTerm
(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Polychlorinated dibenzo-p-dioxins (PCDDs) are pervasive environmental pollutants and resistant in soil and sediment environments. Under anaerobic conditions, PCDDs can undergo reductive dechlorination which produces less chlorinated dibenzo-p-dioxin congeners, these compounds are more susceptible to further degradation. The process is mediated by dehalogenating bacteria, some of which gain energy from the process by using PCDDs as terminal electron acceptors. The objective of our study was to use a conventional enrichment approach to enrich for dehalogenating bacteria, to investigate the PCDD dechlorination activity and the microbial community of enrichment cultures, and to investigate genomes of the abundant dehalogenating bacteria in enrichment cultures from metagenomics data of the corresponding enrichment cultures. PCDD dechlorination was observed in five sets of enrichment cultures established from Hackensack River sediments collected along the tidally influenced river, in which dechlorination activity was negatively affected by high salinity and high sulfate concentrations. Dechlorination activity was also recorded in enrichment cultures established from freshwater sediments of the Kymijoki River (Findland), a water reservoir (Vietnam), as well as from rice paddy soil (Vietnam). Reductive dechlorination of PCDDs was mediated by organohalide-respiring bacteria. PCDD dechlorinating anaerobic bacteria appeared to be ubiquitous, and their activity was observed in almost all enrichment cultures established using soils and sediments collected from sites of different contamination backgrounds. Dehalococcoides spp. were identified as the main bacterial species that couple dechlorination of PCDDs to growth. Genomes of two Dehalococcoides spp. were reconstructed from metagenomes of the Hackensack and the Kymijoki River enrichment cultures in which they were present at relatively high abundance. Comparative genome analysis suggested that a reductive dehalogenase cbrA ortholog is responsible for reductive dechlorination of 1,2,3,4-Tetrachlorodibenzo-p-dioxin in a respiratory process. Genome analysis provided preliminary evidence for the function of reductive dehalogenases in PCDD dechlorination which have not been studied before due to the complex nutritional requirements and slow growth of Dehalococcoides spp.
Subject
(authority = RUETD)
Topic
Microbial Biology
Subject
(authority = ETD-LCSH)
Topic
Soil pollution
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_7603
PhysicalDescription
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(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (xi, 166 p. : ill.)
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Hang Thuy Dam
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3F1920Q
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Dam
GivenName
Hang
MiddleName
Thuy
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
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(qualifier = exact);
(point = start)
2016-09-26 22:14:54
AssociatedEntity
Name
Hang Dam
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
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(qualifier = exact);
(point = start)
2016-10-31
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = end)
2018-10-31
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after October 31st, 2018.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
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ContentModel
ETD
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windows xp
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1.5
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2016-09-27T18:37:01
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2016-09-27T18:37:01
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