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Effect of egg yolk extract on proliferation and myogenic differentiation of C2C12 myoblasts

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TitleInfo
Title
Effect of egg yolk extract on proliferation and myogenic differentiation of C2C12 myoblasts
Name (type = personal)
NamePart (type = family)
Joglekar
NamePart (type = given)
Devika Umesh
NamePart (type = date)
1991-
DisplayForm
Devika Umesh Joglekar
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Freeman
NamePart (type = given)
Joseph W
DisplayForm
Joseph W Freeman
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Olabisi
NamePart (type = given)
Ronke
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Ronke Olabisi
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Berthiaume
NamePart (type = given)
Francois
DisplayForm
Francois Berthiaume
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2016
DateOther (qualifier = exact); (type = degree)
2016-10
CopyrightDate (encoding = w3cdtf); (qualifier = exact)
2016
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Muscle wasting disorders like atrophy and sarcopenia are characterized by compromised skeletal muscle mass and function. One of the many causes of muscle wasting reported is the failure of satellite cells to fuse and form myofibres. Satellite cells are an integral part of skeletal muscle regeneration because of their self-renewal and differentiation abilities. Muscle regeneration research with respect to satellite cells has stimulated interest in discovering factors that enhance proliferation and differentiation as a first step in the regenerative process. The importance of nutrition in regulating muscle mass and muscle protein synthesis is well established. However, limited literature is available regarding the role of nutrition in muscle regeneration; specifically with respect to satellite cells. As for nutrition, chicken egg yolk appears to be a promising candidate. Egg yolks are rich in nutrients such as vitamins, minerals, amino acids and proteins which can provide sustained supply of nutrition to the cells in-vitro. In the present study, we tested the effect of different fractions and concentrations of egg yolk extract on C2C12 myoblasts. For this purpose, egg yolk extract was supplemented in growth media and cells were grown in this nutrient rich media for one week. Cell viability, fluorescence imaging and mRNA expression of muscle specific genes were analyzed at specific time points of proliferation and differentiation stages of myoblast. The study results showed that supplementation of the egg yolk extract in media enhanced proliferation and differentiation of myoblasts in a dose dependent manner which was analyzed by increase in cell viability, number of nuclei, number of myofibres and mRNA expression of muscle specific genes such as MyoD and myogenin.
Subject (authority = RUETD)
Topic
Biomedical Engineering
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_7596
PhysicalDescription
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electronic resource
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application/pdf
InternetMediaType
text/xml
Extent
1 online resource (xi, 52 p. : ill.)
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Devika Umesh Joglekar
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
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NjNbRU
Identifier (type = doi)
doi:10.7282/T3HH6NC0
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Joglekar
GivenName
Devika
MiddleName
Umesh
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2016-09-20 08:26:00
AssociatedEntity
Name
Devika Joglekar
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2016-10-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2017-10-31
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after October 31st, 2017.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical

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2016-09-20T08:24:52
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2016-09-20T08:24:52
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