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Assessing the cytotoxicity of newly developed gliadin nanoparticles loading polymethoxyflavones
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Wang, Xiangan. Assessing the cytotoxicity of newly developed gliadin nanoparticles loading polymethoxyflavones. Retrieved from https://doi.org/doi:10.7282/T3FQ9ZXG

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TitleAssessing the cytotoxicity of newly developed gliadin nanoparticles loading polymethoxyflavones
NameWang, Xiangan (author); HUANG, QINGRONG (chair); HO, CHI-TANG (internal member); Corradini, Maria G. (internal member); Rutgers University; Graduate School - New Brunswick
Date Created2016
Other Date2016-10 (degree)
SubjectFood Science, Nanoparticles
Extent1 online resource (x, 53 p. : ill.)
DescriptionWhether the consumption of dietary supplements is helpful or not significantly depends on the bioavailability of the nutraceuticals. Since nutraceuticals dissolve poorly in aqueous system and undergo rapid and intensive metabolism, they are low in bioavailability. In this thesis, a novel delivery system was developed from gliadin to encapsulate polymethoxyflavones, and the cellular uptake and cytotoxicity of the system were assessed upon Caco-2 colon cancer cell lines. Gliadin is the prolamin from wheat thus is GRAS (generally recognized as safe). The huge production of wheat domestic and oversea makes gliadin easily accessible. Gliadin is not well studied for its formulation of nanoparticles, let alone the physiochemical properties or cytotoxicity of gliadin particles. Polymethoxyflavones (PMF) are flavonoids from citrus peels. Although they have shown health benefits such as anti-cancer and anti-inflammation effects, they are extremely low in bioavailability and hardly dissolve in water. In the experiments, gliadin was extracted from commercial gluten, and its purity was evaluated before further study. The shape and size of 1 mg/ml gliadin in 60% ethyl-ethanol was shown prolate ellipsoid and 157 Ã… by 27 Ã… by 6 Ã… based on small angel X-ray scattering (SAXS). Preliminary trails were carried out to find out favorable conditions for gliadin nanoparticle formulation. The PMF-loaded gliadin nanoparticles were prepared by induced self-assembly. Particles presented spherical morphology shown by atomic force microscopy (AFM). Particles have a diameter around 150nm without any chemical agent to stabilize. The PMF-loaded gliadin nanoparticles demonstrated little, if there is any, cytotoxicity upon human colon cancer cells. In short, gliadin nanoparticles formulated in this study have reasonable loading and very low cytotoxicity and may be used to load lipophilic nutraceuticals other than PMF.
NoteM.S.
NoteIncludes bibliographical references
Noteby Xiangan Wang
Genretheses, ETD graduate
Persistent URLhttps://doi.org/doi:10.7282/T3FQ9ZXG
Languageeng
CollectionGraduate School - New Brunswick Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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