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Assessing the cytotoxicity of newly developed gliadin nanoparticles loading polymethoxyflavones
Descriptive
TitleInfo
Title
Assessing the cytotoxicity of newly developed gliadin nanoparticles loading polymethoxyflavones
Name
(type = personal)
NamePart
(type = family)
Wang
NamePart
(type = given)
Xiangan
NamePart
(type = date)
1991-
DisplayForm
Xiangan Wang
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
HUANG
NamePart
(type = given)
QINGRONG
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QINGRONG HUANG
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
HO
NamePart
(type = given)
CHI-TANG
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CHI-TANG HO
Affiliation
Advisory Committee
Role
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(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Corradini
NamePart
(type = given)
Maria G.
DisplayForm
Maria G. Corradini
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
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NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
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NamePart
Graduate School - New Brunswick
Role
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(authority = RULIB)
school
TypeOfResource
Text
Genre
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theses
OriginInfo
DateCreated
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2016
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(type = degree)
2016-10
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2016
Place
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(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Whether the consumption of dietary supplements is helpful or not significantly depends on the bioavailability of the nutraceuticals. Since nutraceuticals dissolve poorly in aqueous system and undergo rapid and intensive metabolism, they are low in bioavailability. In this thesis, a novel delivery system was developed from gliadin to encapsulate polymethoxyflavones, and the cellular uptake and cytotoxicity of the system were assessed upon Caco-2 colon cancer cell lines. Gliadin is the prolamin from wheat thus is GRAS (generally recognized as safe). The huge production of wheat domestic and oversea makes gliadin easily accessible. Gliadin is not well studied for its formulation of nanoparticles, let alone the physiochemical properties or cytotoxicity of gliadin particles. Polymethoxyflavones (PMF) are flavonoids from citrus peels. Although they have shown health benefits such as anti-cancer and anti-inflammation effects, they are extremely low in bioavailability and hardly dissolve in water. In the experiments, gliadin was extracted from commercial gluten, and its purity was evaluated before further study. The shape and size of 1 mg/ml gliadin in 60% ethyl-ethanol was shown prolate ellipsoid and 157 Ã… by 27 Ã… by 6 Ã… based on small angel X-ray scattering (SAXS). Preliminary trails were carried out to find out favorable conditions for gliadin nanoparticle formulation. The PMF-loaded gliadin nanoparticles were prepared by induced self-assembly. Particles presented spherical morphology shown by atomic force microscopy (AFM). Particles have a diameter around 150nm without any chemical agent to stabilize. The PMF-loaded gliadin nanoparticles demonstrated little, if there is any, cytotoxicity upon human colon cancer cells. In short, gliadin nanoparticles formulated in this study have reasonable loading and very low cytotoxicity and may be used to load lipophilic nutraceuticals other than PMF.
Subject
(authority = RUETD)
Topic
Food Science
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_7558
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (x, 53 p. : ill.)
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
Subject
(authority = ETD-LCSH)
Topic
Nanoparticles
Note
(type = statement of responsibility)
by Xiangan Wang
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3FQ9ZXG
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
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Name
FamilyName
Wang
GivenName
Xiangan
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
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(qualifier = exact);
(point = start)
2016-09-12 10:50:49
AssociatedEntity
Name
Xiangan Wang
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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RULTechMD
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2016-09-12T11:58:25
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