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Development of a continuous-flow, automated microfluidic device for single cell level electroporation
Descriptive
TitleInfo
Title
Development of a continuous-flow, automated microfluidic device for single cell level electroporation
Name
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Zheng
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Mingde
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1987-
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Mingde Zheng
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author
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Zahn
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Jeffrey D.
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Jeffrey D. Zahn
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Advisory Committee
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chair
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Shreiber
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David I.
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David I. Shreiber
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Advisory Committee
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internal member
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NamePart
(type = family)
Tischfield
NamePart
(type = given)
Jay A.
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Jay A. Tischfield
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Advisory Committee
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internal member
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(type = personal)
NamePart
(type = family)
Lin
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(type = given)
Hao
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Hao Lin
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Advisory Committee
Role
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(authority = RULIB)
outside member
Name
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(type = family)
Shan
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(type = given)
Jerry W.
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Jerry W. Shan
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Advisory Committee
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outside member
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Rutgers University
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degree grantor
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Graduate School - New Brunswick
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theses
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2016
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2016-10
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2016
Place
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xx
Language
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eng
Abstract
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Electroporation is a powerful transfection technique that creates transient openings in the cell membrane by applying an electric field, allowing for the intracellular delivery of diagnostic and therapeutic substances. The ability to detect and control the degree of cell membrane permeability plays a key role in determining the size of the delivery payload, while safeguarding the overall cell viability. In order to create a universal electroporation system, this dissertation describes the development of a continuous flow electroporation microdevice that automatically detects, electroporates, and monitors individual cells for changes in permeability and delivery. In contrast to devices that immobilize individual cells for impedance analysis, this work demonstrates the capability to manipulate single cells under flow and real-time analysis of membrane permeabilization before and after electroporation, which dramatically increasing the number of cells which can be electroporated and analyzed. Using an electric circuit model, and Multiphysics computational tools, the key parameters for successful cell membrane permeabilization detection in a flow environment were determined. By varying the electric field parameters, we demonstrate the direct control of cell membrane permeabilization by electrically measuring the electroporation-induced cell membrane impedance change and by optically measuring the delivery of a fluorescent probe. Viability of the electroporated cells following collection also demonstrates a correlation with the applied pulse strength. By extending the device capability to include dynamic pulse adjustment according to the real-time feedback information on cell viability, an intelligent electroporation system capable of potentially maximizing delivery efficiency and cell viability can be thus realized.
Subject
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Topic
Biomedical Engineering
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Title
Rutgers University Electronic Theses and Dissertations
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ETD
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ETD_7429
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electronic resource
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application/pdf
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text/xml
Extent
1 online resource (xxiii, 223 p. : ill.)
Note
(type = degree)
Ph.D.
Note
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Includes bibliographical references
Subject
(authority = ETD-LCSH)
Topic
Electroporation
Note
(type = statement of responsibility)
by Mingde Zheng
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
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NjNbRU
Identifier
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doi:10.7282/T3HD7Z04
Genre
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ETD doctoral
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Rights
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The author owns the copyright to this work.
RightsHolder
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Name
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Zheng
GivenName
Mingde
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Permission or license
DateTime
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2016-07-04 15:32:42
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Name
Mingde Zheng
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Affiliation
Rutgers University. Graduate School - New Brunswick
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Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
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2016-10-31
DateTime
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2018-10-31
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after October 31st, 2018.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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