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Continuous-flow, electrically-triggered, single cell-level electroporation

Descriptive

TypeOfResource
Text
TitleInfo
Title
Continuous-flow, electrically-triggered, single cell-level electroporation
Name (type = personal)
NamePart (type = family)
Zheng
NamePart (type = given)
Mingde
Affiliation
Biomedical Engineering, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (authority = orcid); (authorityURI = http://id.loc.gov/vocabulary/identifiers/orcid.html); (type = personal); (valueURI = http://orcid.org/0000-0002-2058-7887)
NamePart (type = family)
Sherba
NamePart (type = given)
Joseph J.
Affiliation
Biomedical Engineering, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Shan
NamePart (type = given)
Jerry
Affiliation
Mechanical and Aerospace Engineering, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Lin
NamePart (type = given)
Hao
Affiliation
Mechanical and Aerospace Engineering, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Shreiber
NamePart (type = given)
David I.
Affiliation
Biomedical Engineering, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Zahn
NamePart (type = given)
Jeffrey D.
Affiliation
Biomedical Engineering, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (authority = RutgersOrg-Department); (type = corporate)
NamePart
Biomedical Engineering
Name (authority = RutgersOrg-School); (type = corporate)
NamePart
School of Engineering
Name (authority = RutgersOrg-Department); (type = corporate)
NamePart
Mechanical and Aerospace Engineering
Genre (authority = RULIB-FS)
Article, Refereed
Genre (authority = NISO JAV)
Accepted Manuscript (AM)
Note (type = peerReview)
Peer reviewed
OriginInfo
DateIssued (encoding = w3cdtf); (keyDate = yes)
2017
Abstract (type = Abstract)
Electroporation creates transient openings in the cell membrane, allowing for intracellular delivery of diagnostic and therapeutic substances. The degree of cell membrane permeability during electroporation plays a key role in regulating the size of the delivery payload as well as the overall cell viability. A microfl uidic platform offers the ability to electroporate single cells with impedance detection of membrane permeabilization in a high-throughput, continuous-fl ow manner. We have developed a fl ow-based electroporation microdevice that automatically detects, electroporates, and monitors individual cells for changes in permeability and delivery. We are able to achieve the advantages of electrical monitoring of cell permeabilization, heretofore only achieved with trapped or static cells, while processing the cells in a continuous-fl ow environment. We demonstrate the analysis of membrane permeabilization on individual cells before and after electroporation in a continuous-fl ow environment, which dramatically increases throughput. We have confi rmed cell membrane permeabilization by electrically measuring the changes in cell impedance from electroporation and by optically measuring the intracellular delivery of a fl uorescent probe after systematically varying the electric fi eld strength and duration and correlating the pulse parameters to cell viability. We fi nd a dramatic change in cell impedance and propidium iodide (PI) uptake at a pulse strength threshold of 0.87 kV/cm applied for a duration of 1 ms or longer. The overall cell viability was found to vary in a dose dependent manner with lower viability observed with increasing electric fi eld strength and pulse duration. Cell viability was greater than 83% for all cases except for the most aggressive pulse condition (1 kV/cm for 5 ms), where the viability dropped to 67.1%. These studies can assist in determining critical permeabilization and molecular delivery parameters while preserving viability.
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
PhysicalDescription
InternetMediaType
application/pdf
Extent
19 p.
Subject (authority = local)
Topic
Single Cell
Subject (authority = local)
Topic
Electroporation
Subject (authority = local)
Topic
Permeabilization
Subject (authority = local)
Topic
Microfluidics
Subject (authority = local)
Topic
Molecular-transport
Extension
DescriptiveEvent
Type
Citation
DateTime (encoding = w3cdtf)
2017
AssociatedObject
Name
Technology
Type
Journal
Relationship
Has part
Detail
1-11
Identifier (type = volume and issue)
5(1)
Reference (type = url)
http://dx.doi.org/10.1142/S2339547817500017
Extension
DescriptiveEvent
Type
Grant award
AssociatedEntity
Role
Funder
Name
National Science Foundation, Chemical, Bioengineering, Environmental, and Transport Systems (CBET)
AssociatedObject
Type
Grant number
Name
0967598
Extension
DescriptiveEvent
Type
Grant award
AssociatedEntity
Role
Funder
Name
National Science Foundation, Instrument Development for Biological Research (IDBR)
AssociatedObject
Type
Grant number
Name
1353918
RelatedItem (type = host)
TitleInfo
Title
Sherba, Joseph J.
Identifier (type = local)
rucore30228700001
RelatedItem (type = host)
TitleInfo
Title
Zheng, Mingde
Identifier (type = local)
rucore30228800001
RelatedItem (type = host)
TitleInfo
Title
Shan, Jerry
Identifier (type = local)
rucore30227900001
RelatedItem (type = host)
TitleInfo
Title
Lin, Hao
Identifier (type = local)
rucore30228900001
RelatedItem (type = host)
TitleInfo
Title
Shreiber, David I.
Identifier (type = local)
rucore30172200001
RelatedItem (type = host)
TitleInfo
Title
Zahn Jeffrey Collection
Identifier (type = local)
rucore30101200001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3028TZ9
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Rights

RightsDeclaration (AUTHORITY = FS); (ID = rulibRdec0004)
Copyright for scholarly resources published in RUcore is retained by the copyright holder. By virtue of its appearance in this open access medium, you are free to use this resource, with proper attribution, in educational and other non-commercial settings. Other uses, such as reproduction or republication, may require the permission of the copyright holder.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsEvent
Type
Permission or license
AssociatedObject
Type
License
Name
Multiple author license v. 1
Detail
I hereby grant to Rutgers, The State University of New Jersey (Rutgers) the non-exclusive right to retain, reproduce, and distribute the deposited work (Work) in whole or in part, in and from its electronic format, without fee. This agreement does not represent a transfer of copyright to Rutgers.Rutgers may make and keep more than one copy of the Work for purposes of security, backup, preservation, and access and may migrate the Work to any medium or format for the purpose of preservation and access in the future. Rutgers will not make any alteration, other than as allowed by this agreement, to the Work.I represent and warrant to Rutgers that the Work is my original work. I also represent that the Work does not, to the best of my knowledge, infringe or violate any rights of others.I further represent and warrant that I have obtained all necessary rights to permit Rutgers to reproduce and distribute the Work and that any third-party owned content is clearly identified and acknowledged within the Work.By granting this license, I acknowledge that I have read and agreed to the terms of this agreement and all related RUcore and Rutgers policies.
RightsEvent
Type
Embargo
DateTime (point = end); (encoding = w3cdtf)
2018-02-15
Detail
Access to this PDF has been restricted at the publisher's request. It will be publicly available after February 14, 2018.
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Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
Document
CreatingApplication
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2017-04-07T14:11:00
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2017-04-07T14:11:00
ApplicationName
Microsoft Office Word
Version
15.0000
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