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Natural variation of quantitative traits for consolidated bioprocessing of cellulosic ethanol in Neurospora crassa
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Waters, Joshua Cain. Natural variation of quantitative traits for consolidated bioprocessing of cellulosic ethanol in Neurospora crassa. Retrieved from https://doi.org/doi:10.7282/T30G3NWK

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TitleNatural variation of quantitative traits for consolidated bioprocessing of cellulosic ethanol in Neurospora crassa
NameWaters, Joshua Cain (author); Lee, Kwangwon (chair); Klein, Eric (internal member); Lun, Desmond (internal member); Rutgers University; Camden Graduate School
Date Created2017
Other Date2017-05 (degree)
SubjectBiology, Biomass energy
Extent1 online resource (iv, 57 p. : ill.)
DescriptionCurrent methods for converting plant biomass to value-added products such as ethanol are expensive and time consuming, requiring thermochemical pretreatment, enzymatic hydrolysis, microbial fermentation, and product recovery. These steps are classically performed separately with different organisms used for enzyme production, hexose fermentation, and pentose fermentation, which further increases production costs. To achieve cost-effective conversion of lignocellulose to ethanol these steps must be consolidated into a one-step reaction where biomass is hydrolyzed and fermented directly. This consolidated bioprocessing (CBP) requires an organism capable of hydrolytic enzyme production and fermentation of hexose and pentose sugars. The model filamentous fungus Neurospora crassa possesses all of these capabilities, making it a strong candidate for CBP. Therefore, we sought to characterize natural variation among populations of N. crassa and assess if selective breeding would provide a reliable route to generating elite strains for bioethanol production. We observed significant variation in natural and lab generated strains, and demonstrated improvements in fermentation in a single generation. Quantitative trait loci (QTL) analysis pointed to genomic locations underlying the observed phenotypic variance in saccharification of cellulose and fermentation of ethanol within one of the populations. Finally, we demonstrated direct fermentation of untreated biomass (Miscanthus giganteus) by N. crassa, highlighting its potential for CBP and demonstrating that natural strains are more proficient at utilization of biomass than the laboratory reference strain.
NoteM.S.
NoteIncludes bibliographical references
Noteby Joshua Cain Waters
Genretheses, ETD graduate
Persistent URLhttps://doi.org/doi:10.7282/T30G3NWK
Languageeng
CollectionCamden Graduate School Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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