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Natural variation of quantitative traits for consolidated bioprocessing of cellulosic ethanol in Neurospora crassa
Descriptive
TitleInfo
Title
Natural variation of quantitative traits for consolidated bioprocessing of cellulosic ethanol in Neurospora crassa
Name
(type = personal)
NamePart
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Waters
NamePart
(type = given)
Joshua Cain
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Joshua Cain Waters
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(authority = RULIB)
author
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(type = personal)
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(type = family)
Lee
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(type = given)
Kwangwon
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Kwangwon Lee
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Advisory Committee
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chair
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(type = personal)
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(type = family)
Klein
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(type = given)
Eric
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Eric Klein
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Advisory Committee
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internal member
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(type = personal)
NamePart
(type = family)
Lun
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(type = given)
Desmond
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Desmond Lun
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Advisory Committee
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internal member
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Rutgers University
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degree grantor
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Camden Graduate School
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school
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Text
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theses
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2017
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2017-05
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2017
Place
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xx
Language
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eng
Abstract
(type = abstract)
Current methods for converting plant biomass to value-added products such as ethanol are expensive and time consuming, requiring thermochemical pretreatment, enzymatic hydrolysis, microbial fermentation, and product recovery. These steps are classically performed separately with different organisms used for enzyme production, hexose fermentation, and pentose fermentation, which further increases production costs. To achieve cost-effective conversion of lignocellulose to ethanol these steps must be consolidated into a one-step reaction where biomass is hydrolyzed and fermented directly. This consolidated bioprocessing (CBP) requires an organism capable of hydrolytic enzyme production and fermentation of hexose and pentose sugars. The model filamentous fungus Neurospora crassa possesses all of these capabilities, making it a strong candidate for CBP. Therefore, we sought to characterize natural variation among populations of N. crassa and assess if selective breeding would provide a reliable route to generating elite strains for bioethanol production. We observed significant variation in natural and lab generated strains, and demonstrated improvements in fermentation in a single generation. Quantitative trait loci (QTL) analysis pointed to genomic locations underlying the observed phenotypic variance in saccharification of cellulose and fermentation of ethanol within one of the populations. Finally, we demonstrated direct fermentation of untreated biomass (Miscanthus giganteus) by N. crassa, highlighting its potential for CBP and demonstrating that natural strains are more proficient at utilization of biomass than the laboratory reference strain.
Subject
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Topic
Biology
Subject
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Topic
Biomass energy
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Title
Rutgers University Electronic Theses and Dissertations
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ETD_8194
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electronic resource
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application/pdf
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text/xml
Extent
1 online resource (iv, 57 p. : ill.)
Note
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M.S.
Note
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Includes bibliographical references
Note
(type = statement of responsibility)
by Joshua Cain Waters
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TitleInfo
Title
Camden Graduate School Electronic Theses and Dissertations
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rucore10005600001
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NjNbRU
Identifier
(type = doi)
doi:10.7282/T30G3NWK
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
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The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Waters
GivenName
Joshua
MiddleName
Cain
Role
Copyright Holder
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Type
Permission or license
DateTime
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2017-05-04 13:10:39
AssociatedEntity
Name
Joshua Waters
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Copyright holder
Affiliation
Rutgers University. Camden Graduate School
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
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2017-05-31
DateTime
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2019-05-31
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 31st, 2019.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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windows xp
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