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Genomic analysis of polyaromatic hydrocarbon degrading Paenibacillus species
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Al-shiti, Ahmed. Genomic analysis of polyaromatic hydrocarbon degrading Paenibacillus species. Retrieved from https://doi.org/doi:10.7282/T3HD7ZFZ

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TitleGenomic analysis of polyaromatic hydrocarbon degrading Paenibacillus species
NameAl-shiti, Ahmed (author); Häggblom, Max (chair); Rutgers University; Graduate School - New Brunswick
Date Created2017
Other Date2017-05 (degree)
SubjectMicrobial Biology, Hydrocarbons
Extent1 online resource (vii, 49 p. : ill.)
DescriptionPolyaromatic hydrocarbons (PAHs) are a group of complex organic compounds that contain at least two aromatic rings. Some of the PAHs, which have high molecular weight, are known carcinogens. PAHs are one of the most prevalent pollutants in the environment and have two main sources: petroleum products and the incomplete combustion of wood. Several microorganisms are known for the ability to degrade PAHs, either aerobically or anaerobically, such as algae, bacteria, and fungi. The initial step of the aerobic degradation of PAHs is catalyzed by dioxygenase enzymes, which introduces oxygen atoms into the aromatic rings. The first aim of this study was to isolate and identify bacterial species that may have unique enzymes for PAH degradation. Two strains, Paenibacillus validus RD5 and RD6, were isolated from sediment samples obtained from the Logan River, Utah, USA, by aerobic enrichment using biphenyl as the sole carbon source. The second aim was to sequence the RD5 and RD6 genomes and examine transcription of several predicted dioxygenase genes in P. validus RD5 and P. naphthalenovorans PRN1 during growth on naphthalene, biphenyl, dibenzofuran, cinnamate, and benzoate using the reverse transcription polymerase chain reaction (RT-PCR) technique. Based on the RT-PCR results and comparison of the dioxygenase sequences to other enzyme sequences in GenBank hypotheses were formulated about the role of three of the enzymes in biphenyl, naphthalene, and dibenzofuran degradation in the two Paenibacillus strains
NoteM.S.
NoteIncludes bibliographical references
Noteby Ahmed Al-Shiti
Genretheses, ETD graduate
Persistent URLhttps://doi.org/doi:10.7282/T3HD7ZFZ
Languageeng
CollectionGraduate School - New Brunswick Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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