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Negatively supercharging cellulases render them lignin-resistant

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TypeOfResource
Text
TitleInfo
Title
Negatively supercharging cellulases render them lignin-resistant
Name (type = personal)
NamePart (type = family)
Whitehead
NamePart (type = given)
Timothy A.
Affiliation
Michigan State University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Bandi
NamePart (type = given)
Chandra K.
Affiliation
Chemical & Biochemical Engineering, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Berger
NamePart (type = given)
Marissa
Affiliation
Biomedical Engineering, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Park
NamePart (type = given)
Jihyun
Affiliation
Chemical & Biochemical Engineering, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (authority = orcid); (authorityURI = http://id.loc.gov/vocabulary/identifiers/orcid.html); (type = personal); (valueURI = http://orcid.org/0000-0003-3677-6735)
NamePart (type = family)
Chundawat
NamePart (type = given)
Shishir P. S.
Affiliation
Chemical & Biochemical Engineering, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (authority = RutgersOrg-Department); (type = corporate)
NamePart
Chemical & Biochemical Engineering
Name (authority = RutgersOrg-School); (type = corporate)
NamePart
School of Engineering
Name (authority = RutgersOrg-Department); (type = corporate)
NamePart
Biomedical Engineering
Genre (authority = RULIB-FS)
Article, Refereed
Genre (authority = NISO JAV)
Accepted Manuscript (AM)
Note (type = peerReview)
Peer reviewed
OriginInfo
DateIssued (encoding = w3cdtf); (keyDate = yes)
2017
Abstract (type = Abstract)
Non-specific adsorption of cellulases to lignin hinders enzymatic deconstruction of lignocellulosic biomass. Here we tested the hypothesis that negatively supercharging cellulases could reduce lignin inhibition. Computational design was used to negatively supercharge the surfaces of Ruminoclostridium thermocellum family 5 CelE and a CelE-family 3a carbohydrate binding module fusion. Resulting designs maintained the same expression yield, thermal stability, and nearly identical activity on soluble substrate as the wild-type proteins. Four designs showed complete lack of inhibition by lignin but with lower cellulose conversion compared to original enzymes. Increasing salt concentrations could partially rescue the activity of supercharged enzymes, supporting a mechanism of electrostatic repulsion between designs and cellulose. Results showcase a protein engineering strategy to construct highly active cellulases that are resistant to lignin-mediated inactivation, although further work is needed to understand the relationship between negative protein surface potential and activity on insoluble polysaccharides.
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
PhysicalDescription
InternetMediaType
application/pdf
Extent
30 p.
Extension
DescriptiveEvent
Type
Citation
DateTime (encoding = w3cdtf)
2017
AssociatedObject
Name
ACS Sustainable Chemistry & Engineering
Type
Journal
Relationship
Has part
Detail
6247-6252
Identifier (type = volume and issue)
5(7)
Reference (type = url)
http://dx.doi.org/10.1021/acssuschemeng.7b01202
Extension
DescriptiveEvent
Type
Grant award
AssociatedEntity
Role
Funder
Name
National Science Foundation
AssociatedEntity
Role
Originator
Name
Shishir Chundawat and Timothy Whitehead
AssociatedObject
Type
Grant number
Name
1236120
Extension
DescriptiveEvent
Type
Grant award
AssociatedEntity
Role
Funder
Name
National Science Foundation
AssociatedEntity
Role
Originator
Name
Shishir Chundawat
AssociatedObject
Type
Grant number
Name
1604421
Subject (authority = local)
Topic
Carbohydrate binding domain
Subject (authority = local)
Topic
Cellulase
Subject (authority = local)
Topic
Extractive ammonia pretreatment
Subject (authority = local)
Topic
Lignin inhibition
Subject (authority = local)
Topic
Lignocellulosic biomass
Subject (authority = local)
Topic
Protein design
Subject (authority = local)
Topic
Protein supercharging
RelatedItem (type = host)
TitleInfo
Title
Chundawat, Shishir P. S.
Identifier (type = local)
rucore30218100001
RelatedItem (type = host)
TitleInfo
Title
Bandi, Chandra K.
Identifier (type = local)
rucore30236100001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T35X2CT1
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Rights

RightsDeclaration (AUTHORITY = FS); (ID = rulibRdec0004)
Copyright for scholarly resources published in RUcore is retained by the copyright holder. By virtue of its appearance in this open access medium, you are free to use this resource, with proper attribution, in educational and other non-commercial settings. Other uses, such as reproduction or republication, may require the permission of the copyright holder.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsEvent
Type
Permission or license
AssociatedObject
Type
License
Name
Sole author license v. 1
Detail
I hereby grant to Rutgers, The State University of New Jersey (Rutgers) the non-exclusive right to retain, reproduce, and distribute the deposited work (Work) in whole or in part, in and from its electronic format, without fee. This agreement does not represent a transfer of copyright to Rutgers.Rutgers may make and keep more than one copy of the Work for purposes of security, backup, preservation, and access and may migrate the Work to any medium or format for the purpose of preservation and access in the future. Rutgers will not make any alteration, other than as allowed by this agreement, to the Work.I represent and warrant to Rutgers that the Work is my original work. I also represent that the Work does not, to the best of my knowledge, infringe or violate any rights of others.I further represent and warrant that I have obtained all necessary rights to permit Rutgers to reproduce and distribute the Work and that any third-party owned content is clearly identified and acknowledged within the Work.By granting this license, I acknowledge that I have read and agreed to the terms of this agreement and all related RUcore and Rutgers policies.
RightsEvent
Type
Embargo
DateTime (point = end); (encoding = w3cdtf)
2018-05-22
Detail
Access to this PDF has been restricted at the publisher's request. It will be publicly available after May 21, 2018.
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Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
Document
CreatingApplication
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2017-05-22T14:14:27
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2017-05-22T10:15:20
ApplicationName
Mac OS X 10.11.6 Quartz PDFContext
Version
1.4
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