As RNA sequencing (RNA-Seq) becomes more affordable to process, the number of sequencing data has increased at a rapid rate. A major challenge in RNA-Seq experiments is the analysis of large amount of data generated by next-generation sequencing. RNA-Seq data analysis usually requires advanced Linux/Unix experience for software installation and tedious commands. This required skill set hinders scientists who are interested in analyzing RNA-Seq data but do not have Linux/Unix experience. Here, we describe an end to end pipeline for RNA-Seq Data Analysis Suite (RAS), which streamlines and speeds up analysis time for both standard and single-cell and standard RNA-Seq data. In addition, RAS is a standalone web application and contains an interface where Linux/Unix skills are not required for the data analysis. RAS creates a web server, where the user can interact with the pipeline on a browser. In addition, Since since RAS utilizes distributed computing, it uses computational resources efficiently. RAS The pipeline was validated on single-cell RNA-Seq datasets publicly available at the Gene Expression Omnibus (GEO) from the NCBI.
Subject (authority = RUETD)
Topic
Biomedical Engineering
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_7931
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (viii, 43 p. : ill.)
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Subject (authority = ETD-LCSH)
Topic
RNA--Analysis
Subject (authority = ETD-LCSH)
Topic
Bioinformatics
Note (type = statement of responsibility)
by Nikhil Kumar
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
Rutgers University. Graduate School - New Brunswick
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Type
License
Name
Author Agreement License
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.