Staff View
Heterologous biosynthesis of natural product naringenin by co-culture engineering

Descriptive

TypeOfResource
Text
TitleInfo
Title
Heterologous biosynthesis of natural product naringenin by co-culture engineering
Name (type = personal)
NamePart (type = family)
Ganesan
NamePart (type = given)
Vijaydev
Affiliation
Chemical & Biochemical Engineering, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Li
NamePart (type = given)
Zhenghong
Affiliation
Chemical & Biochemical Engineering, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Wang
NamePart (type = given)
Xiaonan
Affiliation
Chemical & Biochemical Engineering, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Zhang
NamePart (type = given)
Haoran
Affiliation
Chemical & Biochemical Engineering, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (authority = RutgersOrg-Department); (type = corporate)
NamePart
Chemical & Biochemical Engineering
Name (authority = RutgersOrg-School); (type = corporate)
NamePart
School of Engineering
Genre (authority = RULIB-FS)
Article, Refereed
Genre (authority = NISO JAV)
Version of Record (VoR)
Note (type = peerReview)
Peer reviewed
OriginInfo
DateIssued (encoding = w3cdtf); (keyDate = yes)
2017
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
PhysicalDescription
InternetMediaType
application/pdf
Extent
7 p.
Extension
DescriptiveEvent
Type
Citation
AssociatedObject
Name
Synthetic and Systems Biotechnology
Type
Journal
Relationship
Has part
Detail
236-242
Reference (type = url)
http://dx.doi.org/10.1016/j.synbio.2017.08.003
Identifier (type = volume and issue)
2(3)
DateTime (encoding = w3cdtf)
2017
Subject (authority = local)
Topic
Co-culture engineering
Subject (authority = local)
Topic
Naringenin
Subject (authority = local)
Topic
Heterologous biosynthesis
Subject (authority = local)
Topic
E. coli
Abstract (type = abstract)
Co-culture engineering is an emerging approach for microbial biosynthesis of a variety of biochemicals. In this study, E. coli-E. coli co-cultures were developed for heterologous biosynthesis of the natural product naringenin. The co-cultures were composed of two independent E. coli strains dedicated to functional expression of different portions of the biosynthetic pathway, respectively. The co-culture biosynthesis was optimized by investigating the effect of carbon source, E. coli strain selection, timing of IPTG induction and the inoculation ratio between the co-culture strains. Compared with the monoculture strategy, the utilization of the designed co-cultures significantly improved the naringenin production, largely due to the reduction of metabolic stress, employment of proper hosts for improving pathway enzyme activities, and flexible adjustment of the relative biosynthetic strength between the coculture strains. The findings of this study extend the applicability of co-culture engineering in complex natural product biosynthesis.
RelatedItem (type = host)
TitleInfo
Title
Zhang, Haoran
Identifier (type = local)
rucore30193200001
RelatedItem (type = host)
TitleInfo
Title
Ganesan, Vijaydev
Identifier (type = local)
rucore30236600001
RelatedItem (type = host)
TitleInfo
Title
Li, Zhenghong
Identifier (type = local)
rucore30236700001
RelatedItem (type = host)
TitleInfo
Title
Wang, Xiaonan
Identifier (type = local)
rucore30206300001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3445QDP
Genre (authority = ExL-Esploro)
Journal Article
Back to the top

Rights

RightsDeclaration (AUTHORITY = FS); (ID = rulibRdec0004); (TYPE = [FS] statement #1)
Copyright for scholarly resources published in RUcore is retained by the copyright holder. By virtue of its appearance in this open access medium, you are free to use this resource, with proper attribution, in educational and other non-commercial settings. Other uses, such as reproduction or republication, may require the permission of the copyright holder.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsEvent
Type
Permission or license
AssociatedObject
Type
License
Name
Multiple author license v. 1
Detail
I hereby grant to Rutgers, The State University of New Jersey (Rutgers) the non-exclusive right to retain, reproduce, and distribute the deposited work (Work) in whole or in part, in and from its electronic format, without fee. This agreement does not represent a transfer of copyright to Rutgers. Rutgers may make and keep more than one copy of the Work for purposes of security, backup, preservation, and access and may migrate the Work to any medium or format for the purpose of preservation and access in the future. Rutgers will not make any alteration, other than as allowed by this agreement, to the Work. I represent and warrant to Rutgers that the Work is my original work. I also represent that the Work does not, to the best of my knowledge, infringe or violate any rights of others. I further represent and warrant that I have obtained all necessary rights to permit Rutgers to reproduce and distribute the Work and that any third-party owned content is clearly identified and acknowledged within the Work. By granting this license, I acknowledge that I have read and agreed to the terms of this agreement and all related RUcore and Rutgers policies.
Back to the top

Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
Document
CreatingApplication
Version
1.7
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2017-08-25T00:02:21
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2017-09-01T11:06:55
ApplicationName
Acrobat Distiller 8.1.0 (Windows)
Back to the top
Version 8.3.13
Rutgers University Libraries - Copyright ©2020