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Identification of molecuar interactions between synthetic dyes and food macromolecules by fluorescence spectroscopy

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TitleInfo
Title
Identification of molecuar interactions between synthetic dyes and food macromolecules by fluorescence spectroscopy
Name (type = personal)
NamePart (type = family)
Qu
NamePart (type = given)
Ziqian
NamePart (type = date)
1992-
DisplayForm
Ziqian Qu
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Ludescher
NamePart (type = given)
Richard
DisplayForm
Richard Ludescher
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2017
DateOther (qualifier = exact); (type = degree)
2017-05
CopyrightDate (encoding = w3cdtf); (qualifier = exact)
2017
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Recent studies have demonstrated that in glycerol, glycerol-water, mono and disaccharide solutions, the fluorescence emission intensity of GRAS synthetic colors exhibits a strong sensitivity to the physical properties of the medium, for example, as the viscosity increases, the fluorescence intensity increases. Although molecular mass, structure and intrinsic rigidity of the macromolecule might play a role in probe sensitivity in physical properties of the system, other aspects such as molecular interactions between the probe and the macromolecule may explain these behaviors, such as hydrogen bonding, electrostatic interactions, hydrophobic interactions, etc. The nature of the effect of macromolecules on the dye molecules are to be identified by fluorescence spectroscopy. The fluorescence emission intensity was measured by titrating five carbohydrates (alginate, amylopectin, carboxymethyl cellulose, chitosan and methylcellulose) and five proteins (gelatin, gliadin, albumin, whey protein and zein) into three GRAS synthetic dyes (allura red, sunset yellow and fast green) using a Fluoromax-3 (Horiba Scientific Inc.). Temperature (20┬░C) and dye concentration were kept constant throughout the study. Anisotropy, ionic strength and temperature studies were also carried out to further explore the nature of the molecular interactions. Results indicate that the fluorescence intensity of fast green is increased by the addition of all water soluble proteins, which are BSA, whey protein and gelatin. It is found that fast green exhibits a very strong binding with the bovine serum albumin molecule, involving electrostatic interactions, hydrogen bonding and hydrophobic interactions. Allura red, as an azo dye, shows less sensitivity to water-soluble protein molecules, while it has hydrophobic interactions with methyl cellulose. Sunset yellow does not show obvious sensitivity to any of the carbohydrates and proteins studied. These results provide insights into the molecular interactions between synthetic dyes and food macromolecules. Therefore, these findings can be used to enhance the performance of these dyes as intrinsic luminescent probes for the physical state of foods and can also contribute to the enhanced detection of synthetic dyes in food products.
Subject (authority = RUETD)
Topic
Food Science
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_7949
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (vi, 70 p. : ill.)
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Subject (authority = ETD-LCSH)
Topic
Coloring matter in food
Note (type = statement of responsibility)
by Ziqian Qu
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3BG2RWJ
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Qu
GivenName
Ziqian
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2017-04-07 16:22:18
AssociatedEntity
Name
Ziqian Qu
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical

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windows xp
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2017-04-27T20:23:40
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2017-04-27T20:23:40
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