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Introgression of genetic resistance to downy mildew (peronospora belbahrii) in a non-model plant species, sweet basil (ocimum basilicum)

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TitleInfo
Title
Introgression of genetic resistance to downy mildew (peronospora belbahrii) in a non-model plant species, sweet basil (ocimum basilicum)
Name (type = personal)
NamePart (type = family)
Pyne
NamePart (type = given)
Robert M.
NamePart (type = date)
1988-
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Robert M. Pyne
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
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Simon
NamePart (type = given)
James
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James Simon
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Advisory Committee
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RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Bonos
NamePart (type = given)
Stacy
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Stacy Bonos
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Wyenandt
NamePart (type = given)
Andrew
DisplayForm
Andrew Wyenandt
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Wehner
NamePart (type = given)
Todd
DisplayForm
Todd Wehner
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
outside member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
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theses
OriginInfo
DateCreated (qualifier = exact)
2017
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2017-05
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2017
Place
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xx
Language
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eng
Abstract (type = abstract)
Sweet basil (Ocimum basilicum) is among the most widely cultivated culinary herbs in the United States, Western Europe and Israel. Despite relative economic importance, breeding and genetic study of this plant species has been largely neglected, rendering its >3 Gbp genome largely unexplored. The deficit in available O. basilicum genetic and genomic resources has been highlighted by a worldwide downy mildew epidemic caused by obligate oomycete Peronospora belbahrii, which has yet to be mediated by disease resistant varieties. The goal of this dissertation research was to narrow the information gap preventing an effective disease resistance breeding response. Specific objectives were to: 1) determine population structure and estimate genetic diversity among a panel of downy mildew resistant and susceptible Ocimum spp. accessions, 2) identify mode of inheritance for resistance to downy mildew in a full-sibling family and 3) construct a linkage map for detection of quantitative trait loci (QTL) associated with DM resistance. A nested, model-based cluster analysis demonstrated three major delineations within the Ocimum genus with additional evidence for cryptic structure, especially within the economically important k1 O. basilicum cluster. Distribution of DM resistance was concentrated outside the k1 O. basilicum cluster with the exception of a single k1 genotype, ‘MRI’. Analysis of downy mildew response across F2 and backcross populations over two years and two locations demonstrated major gene control of downy mildew resistance conferred by MRI. Finally, a restriction site associated DNA sequencing (RADseq) approach facilitated the discovery and mapping of >1,800 single nucleotide polymorphism (SNP) and expressed sequence tag simple sequence repeat (EST-SSR) markers. The resulting genetic map was validated by the detection of a major QTL, dm11.1, which explained 38-55% of the phenotypic variance observed for the MRI x SB22 F2 mapping population. Disomic inheritance of SNP and SSR markers support previous cytological evidence that basil has evolved an allopolyploid genome. Results of this dissertation provide the most robust phylogenetic examination of the Ocimum genus to date, characterization of DM heritability across multiple environments and the first report of genetic/QTL mapping for O. basilicum. A current case study is provided for the feasibility of breeding a non-model plant species using classical genetic theory in combination with modern genomic technologies.
Subject (authority = RUETD)
Topic
Plant Biology
Subject (authority = ETD-LCSH)
Topic
Basil--Diseases and pests
Subject (authority = ETD-LCSH)
Topic
Downy mildew diseases
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
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ETD
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ETD_7998
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electronic resource
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application/pdf
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text/xml
Extent
1 online resource (xiii, 178 p. : ill.)
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Robert M. Pyne
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
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NjNbRU
Identifier (type = doi)
doi:10.7282/T36Q215M
Genre (authority = ExL-Esploro)
ETD doctoral
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Rights

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The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Pyne
GivenName
Robert
MiddleName
M.
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2017-04-12 16:24:15
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Name
Robert Pyne
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Rutgers University. Graduate School - New Brunswick
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2017-05-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2019-05-31
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 31st, 2019.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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