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Multi-scale visualization and characterization of lignocellulosic plant cell wall deconstruction during thermochemical pretreatment

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Text
TitleInfo
Title
Multi-scale visualization and characterization of lignocellulosic plant cell wall deconstruction during thermochemical pretreatment
Name (authority = orcid); (authorityURI = http://id.loc.gov/vocabulary/identifiers/orcid.html); (type = personal); (valueURI = http://orcid.org/0000-0003-3677-6735)
NamePart (type = family)
Chundawat
NamePart (type = given)
Shishir P. S.
Affiliation
Michigan State University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (authority = RutgersOrg-Department); (type = corporate)
NamePart
Chemical & Biochemical Engineering
Genre (authority = RULIB-FS)
Article, Refereed
Genre (authority = NISO JAV)
Accepted Manuscript (AM)
OriginInfo
DateIssued (encoding = w3cdtf); (keyDate = yes)
2011
PhysicalDescription
InternetMediaType
application/pdf
Extent
22 p.
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
Subject (authority = local)
Topic
AFEX
Subject (authority = local)
Topic
Pretreatment
Subject (authority = local)
Topic
Corn stover
Abstract (type = abstract)
Deconstruction of lignocellulosic plant cell walls to fermentable sugars by thermochemical and/or biological means is impeded by several poorly understood ultrastructural and chemical barriers. A promising thermochemical pretreatment called ammonia fiber expansion (AFEX) overcomes the native recalcitrance of cell walls through subtle morphological and physicochemical changes that enhance cellulase accessibility without extracting lignin and hemicelluloses into separate liquid streams. Multi-scale visualization and characterization of Zea mays (i.e., corn stover) cell walls were carried out by laser scanning confocal fluorescence microscopy (LSCM), Raman spectroscopy, atomic force microscopy (AFM), electron microscopy (SEM, TEM), nuclear magnetic resonance (NMR), and electron spectroscopy for chemical analysis (ESCA) to elucidate the mechanism of AFEX pretreatment. AFEX first dissolves, then extracts and, as the ammonia evaporates, redeposits cell wall decomposition products (e.g., amides, arabinoxylan oligomers, lignin-based phenolics) on outer cell wall surfaces. As a result, nanoporous tunnel-like networks, as visualized by 3D-electron tomography, are formed within the cell walls. We propose that this highly porous structure greatly enhances enzyme accessibility to embedded cellulosic microfibrils. The shape, size (10 to 1000 nm), and spatial distribution of the pores depended on their location within the cell wall and the pretreatment conditions used. Exposed pore surface area per unit AFEX pretreated cell wall volume, estimated via TEM-tomogram image analysis, ranged between 0.005 and 0.05 nm<sup>2</sup> per nm<sup>3</sup>. AFEX results in ultrastructural and physicochemical modifications within the cell wall that enhance enzymatic hydrolysis yield by 4-5 fold over that of untreated cell walls.
Note (type = peerReview)
Peer reviewed.
Name (type = personal)
NamePart (type = family)
Donohoe
NamePart (type = given)
Bryon S.
Affiliation
Biosciences Center, National Renewable Energy Laboratory (NREL)
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Sousa
NamePart (type = given)
Leonardo
Affiliation
Michigan State University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Elder
NamePart (type = given)
Thomas
Affiliation
USDA-Forest Service, Southern Research Station
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Agarwal
NamePart (type = given)
Umesh P.
Affiliation
USDA-Forest Service, Forest Products Laboratory
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Lu
NamePart (type = given)
Fachuang
Affiliation
DOE Great Lakes Bioenergy Research Center (GLBRC); University of Wisconsin—Madison
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Ralph
NamePart (type = given)
John
Affiliation
DOE Great Lakes Bioenergy Research Center (GLBRC); University of Wisconsin—Madison
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Himmel
NamePart (type = given)
Michael E.
Affiliation
Biosciences Center, National Renewable Energy Laboratory (NREL)
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Balan
NamePart (type = given)
Venkatesh
Affiliation
Michigan State University; DOE Great Lakes Bioenergy Research Center (GLBRC)
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Dale
NamePart (type = given)
Bruce E.
Affiliation
Michigan State University; DOE Great Lakes Bioenergy Research Center (GLBRC)
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (authority = RutgersOrg-School); (type = corporate)
NamePart
School of Engineering
Extension
DescriptiveEvent
Type
Citation
DateTime (encoding = w3cdtf)
2011
AssociatedObject
Type
Journal
Relationship
Has part
Name
Energy & Environmental Science
Identifier (type = volume and issue)
4
Reference (type = url)
http://dx.doi.org/10.1039/C0EE00574F
Detail
973-984
RelatedItem (type = host)
TitleInfo
Title
Chundawat, Shishir P. S.
Identifier (type = local)
rucore30218100001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3J1062S
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Multiple author license v. 1
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2010-12-14T05:49:49
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