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Effects of developmental exposure to estrogenic endocrine-disrupting chemicals methoxychlor and bisphenol A during fetal and neonatal periods on ovarian folliculogenesis and reproductive parameters in rats

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Title
Effects of developmental exposure to estrogenic endocrine-disrupting chemicals methoxychlor and bisphenol A during fetal and neonatal periods on ovarian folliculogenesis and reproductive parameters in rats
Name (type = personal)
NamePart (type = family)
Yirtici
NamePart (type = given)
Seher
NamePart (type = date)
1988-
DisplayForm
Seher Yirtici
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Uzumcu
NamePart (type = given)
Mehmet
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Mehmet Uzumcu
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Zama
NamePart (type = given)
Aparna M.
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Aparna M. Zama
Affiliation
Advisory Committee
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internal member
Name (type = personal)
NamePart (type = family)
Bagnell
NamePart (type = given)
Carol A.
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Carol A. Bagnell
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Roepke
NamePart (type = given)
Troy A.
DisplayForm
Troy A. Roepke
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2017
DateOther (qualifier = exact); (type = degree)
2017-05
CopyrightDate (encoding = w3cdtf); (qualifier = exact)
2017
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Endocrine-disrupting chemicals (EDCs) are synthetic or natural compounds that can be found in the environment in various forms, such as pesticides [e.g., methoxychlor (MXC)], plasticizers [e.g., bisphenol A (BPA)], or pharmaceutical agents [e.g., diethylstilbestrol (DES), a potent synthetic estrogen that was prescribed to pregnant women between 1940s and 1970s. Previous studies indicated that the ovary is particularly vulnerable to estrogenic EDC exposures during fetal and neonatal periods. To investigate the effects of estrogenic EDCs in two critical developmental windows, we performed two experiments, using timed-pregnant Fisher CDF rats. In Experiment 1, we exposed the dams to 50 µg/kg/day (Low) BPA or 50 mg/kg/day (High) BPA, or vehicle from embryonic day (E) 18 to 21, and the pups from postnatal day (PND) 0 to 7. In Experiment 2, we exposed animals to 0.1µg/kg/day DES, 75 mg/kg/day MXC, 50 mg/kg/day BPA, or vehicle from E11 to PND7. We then examined reproductive parameters, including pubertal age, regularity of reproductive cycles, and follicular composition. Expression of critical ovarian markers, including estrogen receptor 1 (ESR1), Mullerian inhibiting substance (MIS), cytochrome P450 side chain cleavage (P450scc), luteinizing hormone receptor (LHR), and proliferating cell nuclear antigen (PCNA) were also examined. In Experiment 1, we found that there were no changes in age at puberty or estrous cyclicity in the both Low and High BPA-treated females. However, there was a decrease (p < 0.05) in primary follicles in High BPA-treated rats. In addition, there was an increase in atretic follicle numbers (p < 0.05) in the Low and High BPA-treated females suggesting that there was an effect on follicular dynamics. Moreover, a short window of ovarian exposure to BPA did not show any statistically significant effects on expression of ovarian markers except ESR1which was decreased in primary follicles in Low BPA-treated females. In Experiment 2, our results demonstrated that DES- and MXC-treated females had an accelerated onset of puberty and altered estrous cyclicity. Although there was no effect on the litter size, MXC-treated females showed a strong trend towards reduction in litter size (p = 0.07) as compared to control. MXC caused a decrease in steroid hormone levels. DES, MXC, and BPA exposures altered follicular dynamics. There was an increase in atretic follicles in DES and BPA-treated females. In addition, the number of corpora lutea (CL) was reduced (p < 0.01) in MXC-treated females while the total follicle numbers were not altered. There were alterations in ovarian molecular markers. Expression of MIS significantly increased in secondary and pre-antral follicles in MXC-treated group. Furthermore, expression of P450scc decreased in pre- and early-antral follicles in MXC-treated females while the expression increased in CL in BPA-treated females. Overall, the results show that developmental exposure to estrogenic-EDCs affects female reproduction and ovarian follicular dynamics. In addition, DES and MXC can alter the age of puberty, and regulatory of reproductive cycles, which may lead to female fertility problems.
Subject (authority = RUETD)
Topic
Endocrinology and Animal Biosciences
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_7902
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (xii, 94 p. : ill.)
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Subject (authority = ETD-LCSH)
Topic
Pisphenol A--Toxicity
Subject (authority = ETD-LCSH)
Topic
Plastics--Toxicology
Subject (authority = ETD-LCSH)
Topic
Plastics--Environmental aspects
Subject (authority = ETD-LCSH)
Topic
Endocrine disrupting chemicals
Note (type = statement of responsibility)
by Seher Yirtici
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3FT8PZ8
Genre (authority = ExL-Esploro)
ETD graduate
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The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Yirtici
GivenName
Seher
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2017-03-21 09:38:43
AssociatedEntity
Name
Seher Yirtici
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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2017-03-21T15:32:21
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