Staff View
Genome scale tracking of Mycobacterium tuberculosis MazF toxin targets

Descriptive

TitleInfo
Title
Genome scale tracking of Mycobacterium tuberculosis MazF toxin targets
Name (type = personal)
NamePart (type = family)
Greendyk
NamePart (type = given)
Julia Renee
NamePart (type = date)
1993-
DisplayForm
Julia Renee Greendyk
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Woychik
NamePart (type = given)
Nancy A
DisplayForm
Nancy A Woychik
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Bhattacharya
NamePart (type = given)
Debashish
DisplayForm
Debashish Bhattacharya
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Boyd
NamePart (type = given)
Jeffrey M
DisplayForm
Jeffrey M Boyd
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Zylstra
NamePart (type = given)
Gerben J
DisplayForm
Gerben J Zylstra
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
School of Graduate Studies
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2017
DateOther (qualifier = exact); (type = degree)
2017-10
CopyrightDate (encoding = w3cdtf); (qualifier = exact)
2017
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Mycobacterium tuberculosis, the bacteria that causes tuberculosis (TB), claims millions of lives every year. Due to coinfection of TB and HIV, increasing antibiotic resistance, and the unique ability of M. tuberculosis to persist in a non-replicating state within its host as a latent infection, TB eradication attempts have consistently failed. Latent TB infects one-third of the world’s population and is refractory to many antibiotics, yet the molecular mechanisms by which M. tuberculosis enters and maintains latency are poorly understood. However, members of the MazF family of toxins, which act by cleaving specific regions of single-stranded RNA, are implicated in this process. To help elucidate the role that MazF toxins play in latent tuberculosis, we used a novel RNA-sequencing approach, 5’RNA-seq, to determine the RNA targets of 4 M. tuberculosis MazF toxins. While MazF-mt10 cleaved at a specific consensus sequence, CACCU, MazF-mt4, -mt7, and -mt11 did not appear to cleave at a specific sequence. MazF-mt7 cleaved at a number of positions within 23S rRNA; degradation of 23S rRNA could serve as a potent mechanism by which to inhibit translation and modulate growth. MazF-mt7 and -mt10 cleaved 16S rRNA slightly upstream of the 3’ end, thus removing the anti-Shine-Dalgarno sequence and potentially aiding in the formation of stress ribosomes that can preferentially translate leaderless mRNAs in M. tuberculosis. MazF-mt4, -mt7 and –mt11 cleaved distinct tRNAs at their anticodon stem loop, providing another way through which to regulate translation. Results from this study could lead to the identification of MazF-related biomarkers for latent tuberculosis, which could in turn be used to develop new, more effective antimicrobials to fight latent tuberculosis.
Subject (authority = RUETD)
Topic
Microbial Biology
Subject (authority = ETD-LCSH)
Topic
Mycobacterium tuberculosis
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_8315
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (x, 56 p. : ill.)
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Julia Renee Greendyk
RelatedItem (type = host)
TitleInfo
Title
School of Graduate Studies Electronic Theses and Dissertations
Identifier (type = local)
rucore10001600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3K077D9
Genre (authority = ExL-Esploro)
ETD graduate
Back to the top

Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Greendyk
GivenName
Julia
MiddleName
Renee
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2017-08-29 22:04:19
AssociatedEntity
Name
Julia Greendyk
Role
Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2017-10-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2019-10-31
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after October 31st, 2019.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
Back to the top

Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
CreatingApplication
Version
1.4
DateCreated (point = start); (encoding = w3cdtf); (qualifier = exact)
2017-09-13T00:39:44
DateCreated (point = start); (encoding = w3cdtf); (qualifier = exact)
2017-09-13T04:39:23
ApplicationName
Adobe Mac PDF Plug-in
Back to the top
Version 8.3.13
Rutgers University Libraries - Copyright ©2021