Staff View
Rroles of plant defense response and bacterial surface polysaccharides in survival of human enteric pathogens Escherichia coli O157:H7 and Escherichia coli O104:H4 on plants

Descriptive

TitleInfo
Title
Rroles of plant defense response and bacterial surface polysaccharides in survival of human enteric pathogens Escherichia coli O157:H7 and Escherichia coli O104:H4 on plants
Name (type = personal)
NamePart (type = family)
Jang
NamePart (type = given)
Hye In
NamePart (type = date)
1985-
DisplayForm
Hye In Jang
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Matthews
NamePart (type = given)
Karl R
DisplayForm
Karl R Matthews
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
School of Graduate Studies
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2017
DateOther (type = degree); (qualifier = exact)
2017-10
CopyrightDate (encoding = w3cdtf); (qualifier = exact)
2017
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Human enteric pathogens are associated with numerous outbreaks by consumption of contaminated fresh produce, which indicates that plants or vegetable crops can be potential hosts for pathogens. In order to enhance safety of fresh produce, it is important to understand the interactions between human enteric pathogens and plants. However, little information is available about the behavior of human enteric pathogens on plants, such as mechanisms of survival and persistence. In this study, we investigated survival and persistence of Escherichia coli O157:H7 and O104:H4 strains on Arabidopsis thaliana and romaine lettuce, as well as production of capsular polysaccharide (CPS) and induction of plant defense response. Colonization study with E. coli O157:H7 86-24 wild-type strain and its isogenic mutants of surface polysaccharides showed that colanic acid-deficient and lipopolysaccharide (LPS)-deficient mutants significantly less survived on Arabidopsis plant and lettuce on day 1 and 5 post-inoculation, compared to the wild-type. The two mutants of colanic acid and LPS induced 2-fold greater PR1 gene expression and produced significantly lower amount of CPS compared to wild-type strain (P < 0.05). The results may suggest that structures of colanic acid and LPS of E. coli O157:H7 influence the plant defense response, thereby resulting in different survival and colonization on plants. To investigate fitness of an emerging Shiga toxin-producing E. coli (STEC), colonization of E. coli O104:H4 strains on plants were compared with of that of E. coli O157:H7 strains. Results showed that E. coli O104:H4 strains (RG1, C3493, and LpfA) significantly survived better than E. coli O157:H7 strains (7386 and sakai) on Arabidopsis plant and lettuce at day 5, with greater production of CPS and lower expression of PR1 gene (P < 0.05). These results indicate that different level of plant defense response and CPS production may have an impact on survival or fitness of E. coli O104:H4 and O157:H7 on plants. In order to develop control strategies in crop cultivation environments, it is essential to learn about the behavior of human enteric pathogens on plants, particularly factors influencing the ability of pathogen to overcome plant host immunity. The present study provides better understanding of roles of plant defense response and surface polysaccharides on the molecular interactions between human pathogens and plants. Interestingly, the similar trend of bacterial survival/persistence between Arabidopsis (model plant) and lettuce (plant crop) may suggest a potential use of Arabidopsis as an appropriate model plant for studying the mechanisms of plant responses to human enteric pathogens on leafy vegetables. This study also provides an insight into potential roles of CPS in the survival of human enteric pathogens.
Subject (authority = RUETD)
Topic
Food Science
Subject (authority = ETD-LCSH)
Topic
Escherichia coli
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_8401
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (xiii, 93 p. : ill.)
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Hye In Jang
RelatedItem (type = host)
TitleInfo
Title
School of Graduate Studies Electronic Theses and Dissertations
Identifier (type = local)
rucore10001600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T37D2Z7W
Back to the top

Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Jang
GivenName
Hye In
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2017-09-30 17:57:27
AssociatedEntity
Name
HYE IN JANG
Role
Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2017-10-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2018-05-02
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 2nd, 2018.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
Back to the top

Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
OperatingSystem (VERSION = 5.1)
windows xp
CreatingApplication
Version
1.4
ApplicationName
Mac OS X 10.10.5 Quartz PDFContext
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2017-09-30T21:42:05
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2017-09-30T21:42:05
Back to the top
Version 8.3.13
Rutgers University Libraries - Copyright ©2020