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Study of Top2b function on fibroblast migration using microfluidic device
Descriptive
TitleInfo
Title
Study of Top2b function on fibroblast migration using microfluidic device
Name
(type = personal)
NamePart
(type = family)
Shi
NamePart
(type = given)
Jingjing
NamePart
(type = date)
1992-
DisplayForm
Jingjing Shi
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Cai
NamePart
(type = given)
Li
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Li Cai
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Sy
NamePart
(type = given)
Jay C.
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Jay C. Sy
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Zahn
NamePart
(type = given)
Jeffrey D.
DisplayForm
Jeffrey D. Zahn
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
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NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
School of Graduate Studies
Role
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school
TypeOfResource
Text
Genre
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theses
OriginInfo
DateCreated
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2017
DateOther
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2017-10
CopyrightDate
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2017
Place
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(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Background: Top2b is known to be essential in neural development. The lack of Top2b in embryonic stem cell (ESC)-derived neurons may cause premature cell death, as well as abnormal retinal development. So far, few research has focused on using fibroblasts to investigate the function of Top2b. Therefore, function of Top2b in fibroblast migration still remains unclear. Material and methods: Fibroblasts were obtained from postnatal day 1 (P1) mice and cultured for 7 days before transferred to microfluidic devices. Top2b inhibitor ICRF-193 was used to suppress Top2b function and determine the effect of Top2b on fibroblast migration. Fibroblasts were seeded on one side of two-chamber microfluidic devices and treated with 0.1 μM, 1 μM, and 10 μM ICRF-193, whereas equal concentration of DMSO and DMEM were used as controls. Continuous 7-day observation starts from 1 day after seeding cells on devices. Overnight time-lapse microscopy was performed on cells between 2 days and 4 days. Results: Significant differences in the number and speed of fibroblast cell migration were observed with ICRF-193 treatment. Higher concentration of ICRF-193 resulted in fewer cells in the migration chamber and slower migration speed. Conclusions: These results demonstrate an important role of Top2b in fibroblast migration, thus further making Top2b as a potential target for wound healing and treatment of cancer.
Subject
(authority = RUETD)
Topic
Biomedical Engineering
Subject
(authority = ETD-LCSH)
Topic
Cell migration
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
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ETD
Identifier
ETD_8360
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (ix, 41 p. : ill.)
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Jingjing Shi
RelatedItem
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TitleInfo
Title
School of Graduate Studies Electronic Theses and Dissertations
Identifier
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rucore10001600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T30P135V
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Shi
GivenName
Jingjing
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
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(qualifier = exact);
(point = start)
2017-09-18 05:50:36
AssociatedEntity
Name
JINGJING SHI
Role
Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
RULTechMD
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ContentModel
ETD
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windows xp
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1.4
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Mac OS X 10.12.6 Quartz PDFContext
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2017-09-18T09:48:39
DateCreated
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2017-09-18T09:48:39
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