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Screening and characterization of a mutant library of the microalga nannochloropsis oceanica for growth and lipid production at high CO2 conditions
Descriptive
TitleInfo
Title
Screening and characterization of a mutant library of the microalga nannochloropsis oceanica for growth and lipid production at high CO2 conditions
Name
(type = personal)
NamePart
(type = family)
Vu
NamePart
(type = given)
Hoa
NamePart
(type = date)
1985-
DisplayForm
Hoa Vu
Role
RoleTerm
(authority = RULIB)
author
Name
(type = personal)
NamePart
(type = family)
Dismukes
NamePart
(type = given)
Charles
DisplayForm
Charles Dismukes
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
chair
Name
(type = personal)
NamePart
(type = family)
Bhattacharya
NamePart
(type = given)
Debashish
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Debashish Bhattacharya
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Lun
NamePart
(type = given)
Desmond
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Desmond Lun
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
internal member
Name
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(type = corporate)
NamePart
School of Graduate Studies
Role
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(authority = RULIB)
school
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Text
Genre
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theses
OriginInfo
DateCreated
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2017
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(type = degree)
2017-10
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2017
Place
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(type = code)
xx
Language
LanguageTerm
(authority = ISO639-2b);
(type = code)
eng
Abstract
(type = abstract)
Nannochloropsis is a genus of microalgae that produces substantial amounts of storage lipids referred to as triacylglycerides that are derived from fatty acids. These products are precursors to important dietary lipids for use in oils and as replacements for hydrocarbon fuels. To enhance lipid production in Nannochloropsis oceanica CCMP 1779 we applied an insertional mutagenesis approach to produce a library of 1200 strains containing single insertions located randomly in the genome. The collection was screened for growth at high CO2 (≤10%) and low pH, and for high lipid content. Seven mutants were selected for quantitative assays and characterization of photosynthetic efficiency. All 7 strains and the wild type (WT) grow fastest as measured by growth rate and final dry biomass at 2% CO2. The WT had a higher growth rate and biomass production compared with the mutant strains at 2% and 10% CO2. There was pH and CO2-dependence of the growth rate of mutants and the WT. A level of 10% CO2 was a stress condition for Nannocholoropsis oceanica CCMP 1779. One strain (G2) grown at 2% and 10% CO2 had a higher lipid content by Nile Red fluorescence than the WT. When quantified by lipid extraction, this strain did not show a higher lipid content than the WT by day 15 of growth.
Subject
(authority = RUETD)
Topic
Microbial Biology
Subject
(authority = ETD-LCSH)
Topic
Microalgae
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
Identifier
ETD_8403
PhysicalDescription
Form
(authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (vii, 34 p. : ill.)
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references
Subject
(authority = ETD-LCSH)
Topic
Lipids
Note
(type = statement of responsibility)
by Hoa Vu
RelatedItem
(type = host)
TitleInfo
Title
School of Graduate Studies Electronic Theses and Dissertations
Identifier
(type = local)
rucore10001600001
Location
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(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3474F13
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
(ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Vu
GivenName
Hoa
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
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(qualifier = exact);
(point = start)
2017-09-25 22:39:02
AssociatedEntity
Name
Hoa Vu
Role
Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = start)
2017-10-31
DateTime
(encoding = w3cdtf);
(qualifier = exact);
(point = end)
2018-05-02
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 2nd, 2018.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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ETD
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windows xp
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