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Engineering of synthetic biodegradation pathway towards environmental pollutant 1,2,3-trichloropropane
Descriptive
TitleInfo
Title
Engineering of synthetic biodegradation pathway towards environmental pollutant 1,2,3-trichloropropane
Name
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Yang
NamePart
(type = given)
Lu
NamePart
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1988-
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Lu Yang
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author
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Khare
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Sagar D
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Sagar D Khare
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Advisory Committee
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chair
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Burley
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Stephen K
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Stephen K Burley
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Advisory Committee
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internal member
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Baum
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Jean
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Jean Baum
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Advisory Committee
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internal member
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(type = family)
Nanda
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Vikas
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Vikas Nanda
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Advisory Committee
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outside member
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Rutgers University
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degree grantor
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School of Graduate Studies
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theses
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2018
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2018-01
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2018
Place
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xx
Language
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eng
Abstract
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This dissertation describes the application of protein engineering and metabolic engineering approaches for rational redesign of metabolic pathway for biodegradation of important environmental pollutant 1,2,3-trichloropropane (TCP) utilizing both computational and experimental approaches. A yeast surface display based assay was developed for the limiting enzyme in the pathway - haloalkane dehalogeanse DhaA, which enables high-throughput screening for designed mutants and various strategies were attempted enzyme tunnel engineering on haloalkane dehalogenase DhaA to obtain new starting point towards TCP biodegradation including slot tunnel redesign, de novo tunnel design and chimeric protein design with preserved tunnels and generated combinatorial design library subject to high-throuput screening, but unfortunately no design with both decent expression and enhanced activity was detected. Apart from engineering, the TCP degradation pathway was optimized by metabolic engineering. A phosphorylation- and optically-responsive metabolon for the biodegradation of the environmental pollutant 1,2,3-trichloropropane (TCP) was constructed. The pathway efficiency improvement caused by co-localization in response to stimulus was demonstrated. The design method is modular and generalizable, and could enable spatio-temporal control over a wide variety of synthetic biotransformations. To summarize, this dissertation presents an innovative concept for rational engineering of a synthetic biodegradation pathway for pollutant compound 1,2,3-trichloropropane and explores design approaches of protein engineering and metabolic engineering.
Subject
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Topic
Chemistry and Chemical Biology
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Title
Rutgers University Electronic Theses and Dissertations
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ETD_8555
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electronic resource
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application/pdf
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text/xml
Extent
1 online resource (xiv, 108 p. : ill.)
Note
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Ph.D.
Note
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Includes bibliographical references
Subject
(authority = ETD-LCSH)
Topic
Protein engineering
Note
(type = statement of responsibility)
by Lu Yang
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TitleInfo
Title
School of Graduate Studies Electronic Theses and Dissertations
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rucore10001600001
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Identifier
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doi:10.7282/T30R9SMP
Genre
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ETD doctoral
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Rights
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The author owns the copyright to this work.
RightsHolder
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Name
FamilyName
Yang
GivenName
Lu
Role
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RightsEvent
Type
Permission or license
DateTime
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2017-12-09 19:30:10
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Name
Lu Yang
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Affiliation
Rutgers University. School of Graduate Studies
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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2018-01-31
DateTime
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2018-08-02
Type
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Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after August 2nd, 2018.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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2018-01-18T18:16:13
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2018-01-18T18:16:13
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