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Reconstructed genomes of novel Dehalococcoides mccartyi strains from 1,2,3,4-tetrachlorodibenzo-p-dioxin-dechlorinating enrichment cultures reveal divergent reductive dehalogenase gene profiles

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TypeOfResource
Text
TitleInfo
Title
Reconstructed genomes of novel Dehalococcoides mccartyi strains from 1,2,3,4-tetrachlorodibenzo-p-dioxin-dechlorinating enrichment cultures reveal divergent reductive dehalogenase gene profiles
Name (type = personal)
NamePart (type = family)
Dam
NamePart (type = given)
Hang T.
Affiliation
Biochemistry and Microbiology, Rutgers University; Karlsruher Institut für Technologie (Karlsruhe Institute of Technology)
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Vollmers
NamePart (type = given)
John
Affiliation
Karlsruher Institut für Technologie (Karlsruhe Institute of Technology)
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Kaster
NamePart (type = given)
Anne-Kristin
Affiliation
Karlsruher Institut für Technologie (Karlsruhe Institute of Technology)
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (authority = orcid); (authorityURI = http://id.loc.gov/vocabulary/identifiers/orcid.html); (type = personal); (valueURI = http://orcid.org/0000-0001-6307-7863)
NamePart (type = family)
Häggblom
NamePart (type = given)
Max M.
Affiliation
Biochemistry and Microbiology, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (authority = RutgersOrg-Department); (type = corporate)
NamePart
Biochemistry and Microbiology
Name (authority = RutgersOrg-School); (type = corporate)
NamePart
School of Environmental and Biological Sciences (SEBS)
Genre (authority = RULIB-FS)
Article, Refereed
Genre (authority = NISO JAV)
Accepted Manuscript (AM)
Note (type = peerReview)
Peer reviewed
OriginInfo
DateIssued (encoding = w3cdtf); (keyDate = yes); (qualifier = exact)
2017
Abstract (type = Abstract)
Polychlorinated dibenzo-p-dioxin (PCDD) contaminated sites are widespread and associated with a variety of anthropogenic sources. PCDDs and other organohalide pollutants can serve as terminal electron acceptors for anaerobic respiration by specialized bacteria containing reductive dehalogenases (RdhA). These microorganisms, therefore, play an important role in bioremediation of PCDD contaminated sites. Two anaerobic enrichment cultures established using sediments collected from the PCDD polluted Hackensack (USA) and Kymijoki (Finland) rivers showed robust reductive dechlorination of 1,2,3,4-tetrachlorodibenzo-p-dioxin (1,2,3,4-TeCDD). Here, we report on the draft genome reconstructions of the two predominant Dehalococcoides strains from the metagenomes of these dehalogenating enrichment cultures. Furthermore, we gathered a complete list of reductive dehalogenases in the two predominant Dehalococcoides strains, and determined which are likely to be responsible for reductive dechlorination of PCDDs. The divergent rdhA gene profiles of the Dehalococcoides strains likely reflect their exposure to different organohalide compounds in their original habitats. Both draft genomes contained a full length rdhA gene with high sequence similarity to a reductive dehalogenase gene found in Dehalococcoides mccartyi CBDB1 known to reductively dechlorinate 1,2,3,4-tetrachlorobenzene, i.e. cbrA. This gene homologue might also be responsible for reductive dechlorination of 1,2,3,4-TeCDD in the enrichments and could be used as a biomarker to determine the potential for bioremediation of PCDD contaminated sediments.
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
PhysicalDescription
InternetMediaType
application/pdf
Extent
24 p.
Extension
DescriptiveEvent
Type
Citation
DateTime (encoding = w3cdtf); (qualifier = exact)
2017
AssociatedObject
Name
FEMS Microbiology Ecology
Type
Journal
Relationship
Has part
Detail
fix151
Identifier (type = volume and issue)
93(12)
Reference (type = url)
https://doi.org/10.1093/femsec/fix151
Subject (authority = local)
Topic
Dehalococcoides
Subject (authority = local)
Topic
Metagenome binning
Subject (authority = local)
Topic
Chlorinated compounds
Subject (authority = local)
Topic
Polychlorinated dibenzo-p-dioxins
Subject (authority = local)
Topic
Anthropogenic contaminated sediments
Subject (authority = local)
Topic
Bioremediation
RelatedItem (type = host)
TitleInfo
Title
Häggblom, Max M.
Identifier (type = local)
rucore30202400001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3CZ3BDT
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RightsDeclaration (AUTHORITY = FS); (TYPE = [FS] statement #1); (ID = rulibRdec0004)
Copyright for scholarly resources published in RUcore is retained by the copyright holder. By virtue of its appearance in this open access medium, you are free to use this resource, with proper attribution, in educational and other non-commercial settings. Other uses, such as reproduction or republication, may require the permission of the copyright holder.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsEvent
Type
Permission or license
AssociatedObject
Type
License
Name
Multiple author license v. 1
Detail
I hereby grant to Rutgers, The State University of New Jersey (Rutgers) the non-exclusive right to retain, reproduce, and distribute the deposited work (Work) in whole or in part, in and from its electronic format, without fee. This agreement does not represent a transfer of copyright to Rutgers. Rutgers may make and keep more than one copy of the Work for purposes of security, backup, preservation, and access and may migrate the Work to any medium or format for the purpose of preservation and access in the future. Rutgers will not make any alteration, other than as allowed by this agreement, to the Work. I represent and warrant to Rutgers that the Work is my original work. I also represent that the Work does not, to the best of my knowledge, infringe or violate any rights of others. I further represent and warrant that I have obtained all necessary rights to permit Rutgers to reproduce and distribute the Work and that any third-party owned content is clearly identified and acknowledged within the Work. By granting this license, I acknowledge that I have read and agreed to the terms of this agreement and all related RUcore and Rutgers policies.
RightsEvent
Type
Embargo
DateTime (point = end); (encoding = w3cdtf)
2018-11-03
Detail
Access to this PDF has been restricted at the publisher's request. It will be publicly available after November 3, 2018.
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Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
Document
CreatingApplication
Version
1.5
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2018-04-02T11:53:07
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2018-04-02T11:53:07
ApplicationName
Microsoft® Word 2016
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