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Co-aggregation studies of nucleic acid nanostructures with tetracycline molcules
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Title
Co-aggregation studies of nucleic acid nanostructures with tetracycline molcules
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Alzahrani
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Nouf
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Nouf Alzahrani
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author
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Fu
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Jinglin
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Jinglin Fu
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Rutgers University
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degree grantor
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Camden Graduate School
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theses
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2018
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2018-05
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2018
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xx
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eng
Abstract (type = abstract)
Biological systems have evolved complex macromolecular nanostructures to carry out cellular functions with high specificity and efficiency, such as mitochondrial electron transport chains, polymerase and transcription cofactors and light-harvesting antenna in photosynthesis. These organized nanostructures are formed inside cells by spontaneous self-assembly of individual molecular components. In the past few decades, researchers have taken advantage of the self-assembly of nucleic acids to construct various 1D – 3D nanostructures. Self-assembled DNA nanostructures have an inherent advantage of generating programmable nanostructures with controllable parameters of dimension, structural hierarchy and nanometer precision, which can be used for mediating drug release. In this thesis, we have studied single- or double-stranded DNA molecules for forming nanoparticles with minocycline (MH) in the presence of magnesium ions (bridging effect) and π-π stacking interaction. We evaluated multi-dimensional DNA nanostructures (e.g. ssDNA, dsDNA, DNA origami) to load and release MH with sufficient dose during an interval of two weeks. The entrapment efficiency of MH and iii DNA was found to depend on the Mg2+ concentration, DNA length, and types of DNA (i.e. as a function of nitrogen bases). The molecule ssDNA with length > 11-nucleotide (nt) was found to form aggregates with MH in the presence of Mg2+. The titration of Mg2+ concentration showed that the maximum particle formation yield was reached at ~ 4 mM. ssDNA also showed higher dimensional aggregate formation yield than dsDNA, due to the flexible structure of ssDNA allowing more aggregation with MH and Mg2+. In collaboration with Drexel University, we have applied DNA-Mg2+-MH particles to agarose gel encapsulation and release for maintaining the activity of MH during a long period release. This DNA-mediated MH release could be potentially used in the future spinal cord therapy for localized delivery of MH at the injury site.
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Chemistry
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Rutgers University Electronic Theses and Dissertations
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ETD_8998
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electronic resource
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Supplementary File: Figure 2
Extent
1 online resource (x, 37 p. : ill.)
Note (type = degree)
M.S.
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Includes bibliographical references
Subject (authority = ETD-LCSH)
Topic
Nanostructures
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by Nouf Alzahrani
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Camden Graduate School Electronic Theses and Dissertations
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rucore10005600001
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doi:10.7282/T3N58QQV
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ETD graduate
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Rights

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The author owns the copyright to this work.
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Alzahrani
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Nouf
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2018-05-01 10:03:00
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Nouf Alzahrani
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Rutgers University. Camden Graduate School
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Author Agreement License
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Copyright protected
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Open
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